PMID: 6409151Aug 1, 1983Paper

Acyl-CoA synthetase activity of rat heart mitochondria. Substrate specificity with special reference to very-long-chain and isomeric fatty acids

Biochimica Et Biophysica Acta
P T NormannT Flatmark

Abstract

The acyl-CoA synthetase (acid: CoA ligase (AMP-forming), EC 6.2.1.3) activity of rat heart has been measured in fatty acid-depleted fractions of mitochondria, microperoxisomes and microsomes. The assay was based on (i) the measurement of the reaction product AMP by high-performance liquid chromatography or (ii) a coupled reaction in which the intramitochondrial (matrix) CoASH is the final acyl acceptor and the redox state of the flavoproteins in the acyl-CoA dehydrogenase pathway is used to determine the intramitochondrial level of acyl-CoA. This spectrophotometric method was also used to estimate the 'outer' carnitine long-chain acyltransferase (palmitoyl-CoA:L-carnitine O-palmitoyltransferase, EC 2.3.1.21) activity. Comparison of the distribution of long-chain acyl-CoA synthetase activity and marker enzymes in the various subcellular fractions revealed that the synthetase activity is exclusively localized in the mitochondrial fraction. Experimental evidence is presented in support of the conclusion that the chain-length specificity of saturated and monounsaturated fatty acids (16:1-22:1) for the acyl-CoA synthetase is mainly determined by the availability of the fatty acid at the active site, which is largely determined by th...Continue Reading

References

Nov 1, 1978·Journal of Biochemistry·Y Shindo, T Hashimoto
Jan 1, 1977·Progress in the Chemistry of Fats and Other Lipids·J L Beare-Rogers
Jun 1, 1975·Lipids·C K Cheng, S V Pande
Dec 8, 1972·Biochimica Et Biophysica Acta·B O Christophersen, J Bremer
Jun 1, 1974·The Biochemical Journal·R Eisenthal, A Cornish-Bowden

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Citations

Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·R J Henderson, J R Sargent
Dec 4, 2003·Biochimica Et Biophysica Acta·Tal M Lewin, Rosalind A Coleman
Sep 1, 1992·Archives Internationales De Physiologie, De Biochimie Et De Biophysique·C CaselliH Carlier
Dec 1, 1990·Archives internationales de physiologie et de biochimie·C CaselliJ Bezard
May 1, 1988·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·J M MayerB Testa
May 17, 2011·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Ellen DirkxGuillaume J J M van Eys
Aug 4, 1994·Biochimica Et Biophysica Acta·G D LopaschukB O Schönekess
Feb 12, 1986·Biochimica Et Biophysica Acta·N MorisakiS Yoshida
Feb 8, 1985·Biochimica Et Biophysica Acta·N Noy, D Zakim
Oct 1, 1986·Archives of Biochemistry and Biophysics·H Singh, A Poulos

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