PMID: 2110531Mar 26, 1990Paper

Acyl coenzyme A: 6-aminopenicillanic acid acyltransferase from Penicillium chrysogenum and Aspergillus nidulans

FEBS Letters
P A WhitemanA C Willis

Abstract

A study of the final stages of the biosynthesis of the penicillins in Penicillium chrysogenum has revealed two types of enzyme. One hydrolyses phenoxymethyl penicillin to 6-aminopenicillanic acid (6-APA). The other, also obtained from Aspergillus nidulans, transfers a phenylacetyl group from phenylacetyl CoA to 6-APA. The acyltransferase, purified to apparent homogeneity, had a molecular mass of 40 kDa. It also catalyses the conversion of isopenicillin N (IPN) to benzylpenicillin (Pen G) and hydrolyses IPN to 6-APA. In the presence of SDS it dissociates, with loss of activity, into fragments of ca 30 and 10.5 kDa, but activity is regained when these fragments recombine in the absence of SDS.

References

Apr 1, 1988·Natural Product Reports·J E Baldwin, E Abraham
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Oct 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·R H AebersoldS B Kent
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Citations

Jan 1, 1995·Antonie van Leeuwenhoek·J F Martín, S Gutiérrez
Jul 6, 2010·Applied and Environmental Microbiology·Wiebe H MeijerIda J van der Klei
May 28, 2009·BMC Microbiology·Carlos García-EstradaJuan Francisco Martín
Jan 25, 2011·Microbial Biotechnology·Juan F MartínCarlos García-Estrada
Mar 19, 2014·Chemistry & Biology·Shiela E UnklesAxel A Brakhage
Jan 1, 2010·Biotechnology & Genetic Engineering Reviews·Marco Van Den BergIda Vander Keli
Sep 23, 1996·FEBS Letters·P A Whiteman, E P Abraham
Nov 9, 2012·Natural Product Reports·Refaat B HamedChristopher J Schofield
May 15, 2002·Biochimica Et Biophysica Acta·Ted R van der LendeArnold J M Driessen
Sep 8, 1998·Microbiology and Molecular Biology Reviews : MMBR·A A Brakhage

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