PMID: 108099Jan 2, 1979

Acyl-coenzyme-A synthetase I from Candida lipolytica. Purification, properties and immunochemical studies

European Journal of Biochemistry
K HosakaS Numa

Abstract

Acyl-coenzyme-A synthetase I from Candida lipolytica has been purified to homogeneity as evidenced by polyacrylamide gel electrophoresis in the presence and absence of dodecylsulfate as well as by Ouchterlony double-diffusion analysis. The purification procedure involves resolution of cellular particles with Triton X-100 and chromatography on phosphocellulose, Blue-Sepharose and Sephadex G-100. The purified enzyme exhibits a specific activity of 20--24 U/mg protein at 25 degree C, which is about 100-fold higher than those of long-chain acyl-CoA synthetases hitherto reported. The molecular weight of the enzyme has been estimated by polyacrylamide gel electrophoresis in the presence of dodecylsulfate to be approximately 84 000. The enzyme is specific for fatty acids with 14--18 carbon atoms regardless of the degree of unsaturation. Studies with the use of specific antibody to acyl-CoA synthetase I have indicated that this enzyme is immunochemically distinguishable from acyl-CoA synthetase II.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·T KamiryoS Numa
Jan 16, 1978·European Journal of Biochemistry·M MishinaS Numa
Sep 1, 1978·European Journal of Biochemistry·M MishinaS Numa
Dec 1, 1964·The Biochemical Journal·J Bar-Tana, B Shapiro
Feb 1, 1970·European Journal of Biochemistry·D SamuelG AILHAUD
Apr 1, 1971·The Biochemical Journal·J Bar-TanaB Shapiro
Jan 1, 1958·Journal of Neurochemistry·P M VIGNAISI ZABIN
May 1, 1959·Archives of Biochemistry and Biophysics·G L ELLMAN
Nov 4, 1960·Biochimica Et Biophysica Acta·J R SENIOR, K J ISSELBACHER
Nov 5, 1962·Biochimica Et Biophysica Acta·W A CREASEY
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Jan 1, 1949·Acta Pathologica Et Microbiologica Scandinavica·O OUCHTERLONY

Citations

Mar 1, 1988·Molecular and Cellular Endocrinology·E C MandonR R Brenner
Aug 4, 2004·Molecular and Biochemical Parasitology·David W JiangPaul T Englund
Jul 1, 1979·European Journal of Biochemistry·T TanakaS Numa
Feb 15, 1989·European Journal of Biochemistry·E C Webb
May 15, 1996·European Journal of Biochemistry·M KonoY Seyama
Apr 1, 2008·Experimental Biology and Medicine·Eric Soupene, Frans A Kuypers
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T KamiryoS Numa
May 24, 2003·Applied Microbiology and Biotechnology·Y WachéJ-M Belin
May 1, 1983·Neurochemical Research·C Trevisan, S DiMauro
Feb 19, 2013·Journal of Basic Microbiology·Qianqian GaoXuefeng Lu
Mar 1, 1982·Journal of Neurochemistry·M G Murphy, M W Spence
Dec 1, 1996·European Journal of Biochemistry·H IijimaT Yamamoto
May 18, 2004·The Journal of Biological Chemistry·Yuko HisanagaMasashi Miyano

Related Concepts

Candida utilis
Coenzyme A Ligases
Immunodiffusion Measurement
Immunoassay Method
Alloenzymes
Substrate Specificity

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Incretins

Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.