Acyl-coenzyme A:cholesterol acyltransferase-2 (ACAT-2) is responsible for elevated intestinal ACAT activity in diabetic rats

Arteriosclerosis, Thrombosis, and Vascular Biology
Masaharu HoriS Horiuchi

Abstract

Diabetes-induced dyslipidemia is seen in streptozotocin-induced diabetic rats. This is caused, in part, by elevated intestinal acyl-coenzyme A:cholesterol acyltransferase (ACAT) activity. Because two ACAT isozymes (ACAT-1 and ACAT-2) were identified, in the present study we determined which ACAT isozyme was involved in the elevated intestinal ACAT activity in diabetic rats. We cloned a full-length cDNA of rat ACAT-2. Its overexpression in ACAT-deficient AC29 cells demonstrated that the ACAT activity is derived from the cloned cDNA, and a 45-kDa protein of rat ACAT-2 cross-reacts with an anti-human ACAT-2 antibody. The tissue distribution of rat ACAT-2 mRNA revealed its restricted expression to liver and small intestine. Immunohistochemical analyses using an anti-human ACAT-2 antibody demonstrated that ACAT-2 is localized in villus-crypt axis of rat small intestine. The intestinal ACAT activity in diabetic rats was significantly immunodepleted by an anti-ACAT-2 antibody but not by an anti-ACAT-1 antibody. Finally, intestinal ACAT-2 in diabetic rats significantly increased at both protein and mRNA levels as compared with that in control rats. Our data demonstrate that ACAT-2 isozyme is responsible for the increased intestinal ACA...Continue Reading

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Citations

Jan 1, 2009·American Journal of Physiology. Endocrinology and Metabolism·Aaron R Turkish, Stephen L Sturley
Sep 18, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Kylie KavanaghJanice D Wagner
Jul 20, 2012·Journal of Lipid Research·Chang Xie 谢畅Bao-Liang Song 宋保亮
Jul 18, 2008·Experimental Animals·Takashi OkamotoToshiki Sudo
Dec 31, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Muhammad IrfanMan Hee Rhee

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