PMID: 3753462Jun 1, 1986Paper

Acylation of 1-alkenyl-glycerophosphocholine and 1-acyl-glycerophosphocholine in guinea pig heart

The Biochemical Journal
G Arthur, P C Choy

Abstract

The deacylation-reacylation process has been shown to be an important pathway for phospholipids to attain the desired acyl groups at the C-2 position. The acylation of 1-acyl-glycerophosphocholine (-GPC) in mammalian hearts has been well documented, but the acylation of 1-alkenyl-GPC has not been described. In this paper, we demonstrate the presence of acyl-CoA: 1-alkenyl-GPC acyltransferase for the acylation of 1-alkenyl-GPC in mammalian hearts; the highest activity is found in guinea pig heart. The guinea pig heart 1-alkenyl-GPC acyltransferase has only 10-40% of the 1-acyl-GPC acyltransferase activity, and both activities are located in the microsomal fraction. However, these two enzymes respond differently to cations, detergents and heat treatment, and the two enzymes also display different acyl specificity. Kinetic studies indicate that both reactions could not be accommodated by the same catalytic site. The results provide strong evidence that the two activities are from separate and distinct proteins. The specificity of 1-alkenyl-GPC acyltransferase for unsaturated species of acyl-CoA may play an important role in the maintenance of the high degree of unsaturated acyl groups found in guinea pig heart plasmalogens.

Citations

Feb 15, 1989·European Journal of Biochemistry·E C Webb
Dec 25, 2007·Proceedings of the National Academy of Sciences of the United States of America·Eric SoupeneFrans A Kuypers
Aug 4, 1999·Critical Reviews in Clinical Laboratory Sciences·E Kaiser
Nov 25, 1997·Biochimica Et Biophysica Acta·P C ChoyF T Jay
Apr 1, 1997·Archives of Biochemistry and Biophysics·P I Castagnet, N M Giusto
May 23, 1998·Biochimica Et Biophysica Acta·V W Dolinsky, G M Hatch
Jan 5, 1996·Biochimica Et Biophysica Acta·F H ChiltonJ D Winkler
Jun 13, 1998·Progress in Lipid Research·P C ChoyE A Kroeger
May 22, 1988·Biochimica Et Biophysica Acta·M BouroudianG Nalbone
Dec 1, 1994·Chemistry and Physics of Lipids·F Paltauf
Aug 1, 1991·Archives of Biochemistry and Biophysics·T C LeeF Snyder
Oct 1, 1990·Clinical Biochemistry·E KaiserK Zaky

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