PMID: 640849Jan 1, 1978Paper

Acylation of hemoglobin by glutarylsalicylamide and its effect on oxygen transport properties

Hemoglobin
J W Tam

Abstract

Hemoglobin A was modified in vitro with 0.02-0.03 M glutarylsalicylamide for two hours at pH 7.2 and 37 degrees C. The extent of modification was about 30-50%, as estimated by visual comparison after electrophoretic separation. A substantial decrease in oxygen affinity of modified hemoglobin solutions was observed. Similar results were also obtained for dilute cell suspensions of washed red blood cells and whole blood after GSM modification. Other properties such as cooperativity, Bohr effect and 2,3-DPG dependence remained essentially unchanged. Athough the site(s) of modification have not been determined, it is unlikely that they would involve any amino acid residue contributing to the above allosteric properties.

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Citations

Jan 1, 1981·Comparative Biochemistry and Physiology. C: Comparative Pharmacology·J W Tam
Jun 12, 2014·PloS One·Dieter BöningDoris Staab
Mar 1, 1983·Vox Sanguinis·F DeVenuto

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