ADAM10 Sheddase Activity is a Potential Lung-Cancer Biomarker

Journal of Cancer
Toshie YoneyamaNikola L Vujanovic

Abstract

Background: Increases in expression of ADAM10 and ADAM17 genes and proteins are inconsistently found in cancer lesions, and are not validated as clinically useful biomarkers. The enzyme-specific proteolytic activities, which are solely mediated by the active mature enzymes, directly reflect enzyme cellular functions and might be superior biomarkers than the enzyme gene or protein expressions, which comprise the inactive proenzymes and active and inactivated mature enzymes. Methods: Using a recent modification of the proteolytic activity matrix analysis (PrAMA) measuring specific enzyme activities in cell and tissue lysates, we examined the specific sheddase activities of ADAM10 (ADAM10sa) and ADAM17 (ADAM17sa) in human non-small cell lung-carcinoma (NSCLC) cell lines, patient primary tumors and blood exosomes, and the noncancerous counterparts. Results: NSCLC cell lines and patient tumors and exosomes consistently showed significant increases of ADAM10sa relative to their normal, inflammatory and/or benign-tumor controls. Additionally, stage IA-IIB NSCLC primary tumors of patients who died of the disease exhibited greater increases of ADAM10sa than those of patients who survived 5 years following diagnosis and surgery. In contr...Continue Reading

Citations

Oct 27, 2018·Journal of Cellular Biochemistry·Keqiu JiangLiming Wang
Nov 28, 2019·Cancer Metastasis Reviews·Alakesh DasIrit Sagi
Nov 11, 2020·Journal of Hematology & Oncology·Le ZhuQiong-Zhu Dong
Feb 7, 2021·Journal of Molecular Histology·Qinghua FuHongwei Pan
Jun 23, 2021·European Journal of Medicinal Chemistry·Sanjib DasTarun Jha

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Methods Mentioned

BETA
FRET
FCS
density-gradient centrifugation
Fluorescence
ELISA
xenografts

Software Mentioned

PrAMA
Syntherror
R
Matlab
SPSS

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