ADAM23 is downregulated in side population and suppresses lung metastasis of lung carcinoma cells

Cancer Science
Masahide OtaYasunori Okada

Abstract

Cancer cells contain a small population of cancer stem cells or cancer initiating cells, which can be enriched in the side population (SP) after fluorescence activated cell sorting. To examine the members of the ADAM, ADAMTS and MMP gene families related to phenotypes of the SP and the main population (MP), we screened the expression of all the members in the propagated SP and MP of A549 lung adenocarcinoma cells, and found that the relative expression ratio of ADAM23 in the MP to the SP is most highly increased, but none of them are increased in the SP. A similar result on the ADAM23 expression was obtained with another cell line, Calu-3 cells. Overexpression of ADAM23 inhibited colony formation, cell adhesion and migration, and knockdown of ADAM23 by shRNA showed the reverse effects. ADAM23-mediated suppression of colony formation, cell adhesion and migration was greatly reduced by treatment with neutralizing anti-ADAM23 antibody, anti-αvβ3 integrin antibody and/or ADAM23 disintegrin peptide. Expression of cancer stem cell-related genes, including AKRC1/2, TM4SF1 and NR0B1, was increased by knockdown of ADAM23. In addition, lung metastasis of A549 transfectants with different levels of ADAM23 expression was negatively regulat...Continue Reading

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Citations

Nov 4, 2016·Molecular and Cellular Biochemistry·Fei-Fei WangJing-Liang Cheng
Jul 4, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Yang ZhouZhi-Li Liu
Jul 29, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Juan XiaoSuhua Chen
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Oct 6, 2020·Frontiers in Molecular Biosciences·Fangmei FuShiwu Zhang
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May 6, 2021·Journal of Cellular and Molecular Medicine·Chao LiDidi Zuo
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Jul 27, 2021·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Anamarija HabičBarbara Breznik

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Methods Mentioned

BETA
FACS
transfection
PCR
xenografts

Software Mentioned

xCELL
LIVING IMAGE
NIH imageJ

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