Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains

Scientific Reports
Julio Plaza-DíazLuis Fontana

Abstract

We have previously reported that administration of Lactobacillus paracasei CNCM I-4034, Bifidobacterium breve CNCM I-4035 and Lactobacillus rhamnosus CNCM I-4036 to obese Zucker-Lepr fa/fa rats attenuates liver steatosis and exerts anti-inflammatory effects. The goal of the present work was to investigate the modulation of gene expression in intestinal mucosa samples of obese Zucker-Lepr fa/fa rats fed the probiotic strains using a DNA microarray and postgenomic techniques. We also measured secretory IgA content in the gut and lipopolysaccharide (LPS)-binding protein (LBP) in serum. Expression of three genes (Adamdec1, Ednrb and Ptgs1/Cox1) was up-regulated in the intestinal mucosa of the obese rats compared with that in the rats when they were still lean. Probiotic administration down-regulated expression of Adamdec1 and Ednrb at the mRNA and protein levels and that of Ptgs1/Cox1 at the mRNA level, and this effect was in part mediated by a decrease in both macrophage and dendritic cell populations. Probiotic treatment also increased secretory IgA content and diminished the LBP concentration. Based on results reported in this work and else where, we propose a possible mechanism of action for these bacterial strains.

References

Apr 29, 1998·International Journal of Food Microbiology·F Guarner, G J Schaafsma
Dec 4, 2002·JAMA : the Journal of the American Medical Association·Hanna-Maaria LakkaJukka T Salonen
May 26, 2004·Endocrinology and Metabolism Clinics of North America·Gerald Reaven
Dec 8, 2010·Pediatric Research·Natalie S DykstraDavid R Mack
Feb 11, 2012·Canadian Journal of Physiology and Pharmacology·Ana C ZarpelonWaldiceu A Verri
Oct 6, 2012·Annals of Nutrition & Metabolism·Miriam Bermudez-BritoAngel Gil
Feb 13, 2013·The British Journal of Nutrition·Luis FontanaAngel Gil
Aug 31, 2013·Metabolism: Clinical and Experimental·Viswanathan SaraswathiDale S Edgerton
Dec 3, 2014·Current Opinion in Biotechnology·Patrice D Cani, Matthias Van Hul
Jan 17, 2016·Advances in Nutrition·Lena K BraheLesli H Larsen
Oct 21, 2016·European Journal of Nutrition·Mahalingam BalakumarMuthuswamy Balasubramanyam

❮ Previous
Next ❯

Citations

May 8, 2018·Journal of Clinical Gastroenterology·Arthur C OuwehandMichel R Messora
Feb 6, 2019·Advances in Nutrition·Julio Plaza-DiazAngel Gil

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE73848

Methods Mentioned

BETA
PCR
Protein Assay
confocal microscopy
enzyme-linked immunosorbent assay

Software Mentioned

RT 2 Profiler PCR Array Data Analysis
ImageJ
SPSS ( Statistical Package for the Social Sciences ) Statistics
Partek Genomics Suite

Related Concepts

Related Feeds

Anti-inflammatory Treatments

A drug or substance that reduces inflammation (redness, swelling, and pain) in the body. Anti-inflammatory agents block certain substances in the body that cause inflammation and swelling. Discover the latest research on anti-inflammatory treatments here