ADAMTS expression in colorectal cancer

PloS One
Serafula FilouDemitrios H Vynios

Abstract

ADAMTSs are a family of secreted proteinases that share the metalloproteinase domain with matrix metalloproteinases (MMPs). By acting on a large panel of extracellular substrates, they control several cell functions such as fusion, adhesion, proliferation and migration. Through their thrombospondin motifs they also possess anti-angiogenic properties. We investigated whether ADAMTSs participate in colorectal cancer progression and invasion. Their expression was investigated at both mRNA and protein levels. Using RT-PCR, the expression of ADAMTS-1, -4, -5 and ADAMTS-20 was estimated in colorectal tumors of different cancer stage and anatomic site and 3 cell lines of different aggressiveness. An overexpression of ADAMTS-4 and -5 was observed, especially in tissue samples, whereas ADAMTS-1 and -20 were found to be down-regulated. Western blot analysis further supported the RT-PCR findings, revealing in addition the degradation of ADAMTS-1 and -20 in cancer. In situ expression and localization of ADAMTS-1, -4, -5 and -20 was also investigated by immunohistochemical analysis. Our data suggest a positive correlation between ADAMTS-4 and -5 expression and cancer progression, in contrast with the anti-angiogenic members of the family, A...Continue Reading

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Citations

Jan 17, 2017·Journal of Cellular Physiology·Abdullah MoridikiaJafar Salimian
Apr 24, 2019·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Aytekin TokmakArzu Kosem
Nov 30, 2019·Journal of Obstetrics and Gynaecology : the Journal of the Institute of Obstetrics and Gynaecology·Ercan YilmazSerhat Toprak
Mar 5, 2020·Revista da Associação Médica Brasileira·Xue-Qin ShangJing Wu
May 28, 2020·International Journal of Molecular Sciences·Eva AndreuzziMaurizio Mongiat
Sep 10, 2020·OncoTargets and Therapy·Kun ZhangGuiying Wang
Feb 14, 2019·Scientific Reports·Rasha H DoshChristine L Le Maitre
Apr 4, 2021·International Journal of Molecular Sciences·Matej VizovisekSimone Schuerle

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Methods Mentioned

BETA
electrophoresis
X-ray

Software Mentioned

PerlPrimer

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