Adaptable stirred-tank culture strategies for large scale production of multicellular spheroid-based tumor cell models

Journal of Biotechnology
Vítor E SantoCatarina Brito

Abstract

Currently there is an effort toward the development of in vitro cancer models more predictive of clinical efficacy. The onset of advanced analytical tools and imaging technologies has increased the utilization of spheroids in the implementation of high throughput approaches in drug discovery. Agitation-based culture systems are commonly proposed as an alternative method for the production of tumor spheroids, despite the scarce experimental evidence found in the literature. In this study, we demonstrate the robustness and reliability of stirred-tank cultures for the scalable generation of 3D cancer models. We developed standardized protocols to a panel of tumor cell lines from different pathologies and attained efficient tumor cell aggregation by tuning hydrodynamic parameters. Large numbers of spheroids were obtained (typically 1000-1500 spheroids/mL) presenting features of native tumors, namely morphology, proliferation and hypoxia gradients, in a cell line-dependent mode. Heterotypic 3D cancer models, based on co-cultures of tumor cells and fibroblasts, were also established in the absence or presence of additional physical support from an alginate matrix, with maintenance of high cell viability. Altogether, we demonstrate th...Continue Reading

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Citations

Jul 3, 2016·Drug Discovery Today·Maria Chatzinikolaidou
Dec 15, 2016·Biotechnology Journal·Vítor E SantoCatarina Brito
Jan 19, 2018·International Journal of Molecular Sciences·Jessica Hoarau-VéchotJennifer Pasquier
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Jun 21, 2020·International Journal of Molecular Sciences·Shu-Wei HuangFeng-Huei Lin
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Jul 18, 2018·International Journal of Molecular Sciences·Ricardo CoelhoSara Ricardo
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Feb 1, 2021·Cancer Treatment and Research Communications·Azam Bozorgi, Leila Sabouri
Mar 7, 2021·International Journal of Molecular Sciences·Nuno LopesCatarina Brito
May 10, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Dinesh DhamechaJyothi U Menon
Jun 29, 2021·Frontiers in Bioengineering and Biotechnology·Teresa Franchi-MendesCatarina Brito
Jul 3, 2021·Cancers·Fabrizio FontanaPatrizia Limonta
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Paula Rossini AugustiAna Teresa Serra

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