Adaptation by Loss of Heterozygosity in Saccharomyces cerevisiae Clones Under Divergent Selection.

Genetics
Timothy Y JamesShuhua Ge

Abstract

Loss of heterozygosity (LOH) is observed during vegetative growth and reproduction of diploid genotypes through mitotic crossovers, aneuploidy caused by nondisjunction, and gene conversion. We aimed to test the role that LOH plays during adaptation of two highly heterozygous Saccharomyces cerevisiae genotypes to multiple environments over a short time span in the laboratory. We hypothesized that adaptation would be observed through parallel LOH events across replicate populations. Using genome resequencing of 70 clones, we found that LOH was widespread with 5.2 LOH events per clone after ∼500 generations. The most common mode of LOH was gene conversion (51%) followed by crossing over consistent with either break-induced replication or double Holliday junction resolution. There was no evidence that LOH involved nondisjunction of whole chromosomes. We observed parallel LOH in both an environment-specific and environment-independent manner. LOH largely involved recombining existing variation between the parental genotypes, but also was observed after de novo, presumably beneficial, mutations occurred in the presence of canavanine, a toxic analog of arginine. One highly parallel LOH event involved the ENA salt efflux pump locus on ...Continue Reading

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Citations

Jul 31, 2020·G3 : Genes - Genomes - Genetics·Ajith V PankajamKoodali T Nishant
Nov 22, 2020·Molecular Biology and Evolution·Duong T NguyenWeilong Hao
Oct 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Yang SuiThomas D Petes

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