Adaptation in toxic environments: comparative genomics of loci carrying antibiotic resistance genes derived from acid mine drainage waters

Environmental Science and Pollution Research International
Florence Arsène-PloetzeValérie Barbe

Abstract

Several studies have suggested the existence of a close relationship between antibiotic-resistant phenotypes and resistance to other toxic compounds such as heavy metals, which involve co-resistance or cross-resistance mechanisms. A metagenomic library was previously constructed in Escherichia coli with DNA extracted from the bacterial community inhabiting an acid mine drainage (AMD) site highly contaminated with heavy metals. Here, we conducted a search for genes involved in antibiotic resistance using this previously constructed library. In particular, resistance to antibiotics was observed among five clones carrying four different loci originating from CARN5 and CARN2, two genomes reconstructed from the metagenomic data. Among the three CARN2 loci, two carry genes homologous to those previously proposed to be involved in antibiotic resistance. The third CARN2 locus carries a gene encoding a membrane transporter with an unknown function and was found to confer bacterial resistance to rifampicin, gentamycin, and kanamycin. The genome of Thiomonas delicata DSM 16361 and Thiomonas sp. X19 were sequenced in this study. Homologs of genes carried on these three CARN2 loci were found in these genomes, two of these loci were found in...Continue Reading

References

May 28, 2003·Water Research·Corinne CasiotFrançoise Elbaz-Poulichet
Aug 13, 2003·Journal of Applied Microbiology·O BruneelP A D Grimont
Nov 13, 2004·FEMS Microbiology Reviews·Stefan SchwarzAxel Cloeckaert
Feb 1, 2005·Trends in Microbiology·Ronald S Oremland, John F Stolz
Aug 9, 2005·The Science of the Total Environment·C CasiotF Elbaz-Poulichet
Sep 1, 2005·Journal of Industrial Microbiology & Biotechnology·Simon Silver, Le T Phung
Dec 13, 2005·Antonie van Leeuwenhoek·Fabienne Battaglia-BrunetPhilippe Baranger
Jan 5, 2006·Applied and Environmental Microbiology·O BruneelJ-C Personné
Jan 13, 2006·Nucleic Acids Research·David VallenetClaudine Médigue
Mar 16, 2006·Trends in Microbiology·Craig Baker-AustinJ V McArthur
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
Oct 31, 2008·Talanta·Badal Kumar Mandal, Kazuo T Suzuki
Jun 25, 2009·BMC Microbiology·Christopher G BryanFlorence Arsène-Ploetze
Jan 14, 2010·BMC Evolutionary Biology·Alexis DereeperGuillaume Blanc
Feb 17, 2010·Database : the Journal of Biological Databases and Curation·D VallenetC Médigue
Mar 3, 2010·PLoS Genetics·Florence Arsène-PloetzePhilippe N Bertin
Feb 1, 2011·Bioinformatics·Mitchell J SullivanScott A Beatson
Sep 29, 2011·Journal of Basic Microbiology·Sarita MallikAtul Kumar Johri
Apr 7, 2012·Scientific Reports·François DelavatDidier Lièvremont
Jun 21, 2012·Extremophiles : Life Under Extreme Conditions·A VolantO Bruneel
May 8, 2013·Antimicrobial Agents and Chemotherapy·Andrew G McArthurGerard D Wright
Jul 19, 2013·The Science of the Total Environment·François DelavatDidier Lièvremont
Dec 24, 2014·Nature Reviews. Microbiology·José L MartínezFernando Baquero
Aug 11, 2015·Protein Science : a Publication of the Protein Society·Jared A DelmarEdward W Yu
Oct 1, 2015·PloS One·Kelle C FreelFlorence Arsène-Ploetze
Oct 2, 2015·International Journal of Molecular Sciences·Songcan ChenJun Ye
Jan 16, 2016·Environmental Microbiology·Sandrine KoechlerDavid Halter

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Citations

Sep 14, 2018·International Journal of Microbiology·Stephen T Odonkor, Kennedy K Addo
Oct 10, 2020·Microbial Genomics·Kelle C FreelFlorence Arsène-Ploetze
Oct 4, 2020·Applied and Environmental Microbiology·Denise M AkobClara S Chan

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