Adaptation of adherent-invasive E. coli to gut environment: Impact on flagellum expression and bacterial colonization ability.

Gut Microbes
Gwladys SevrinNicolas Barnich

Abstract

The pathogenesis of Crohn's disease (CD) is multifactorial and involves genetic susceptibility, environmental triggers and intestinal microbiota. Adherent-invasive Escherichia coli (AIEC) are flagellated bacteria more prevalent in CD patients than in healthy subjects and promote chronic intestinal inflammation. We aim at deciphering the role of flagella and flagellin modulation by intestinal conditions. AIEC flagellum expression is required for optimal adhesion to and invasion of intestinal epithelial cells. Interestingly, differential flagellin regulation was observed between commensal E. coli (HS) and AIEC (LF82) strains: flagellum expression by AIEC bacteria, in contrast to that of commensal E. coli, is enhanced under intestinal conditions (the presence of bile acids and mucins). Flagella are involved in the ability of the AIEC LF82 strain to cross a mucus layer in vitro and in vivo, conferring a selective advantage in penetrating the mucus layer and reaching the epithelial surface. In a CEABAC10 mouse model, a non-motile mutant (LF82-ΔfliC) exhibits reduced colonization that is restored by a dextran sodium sulfate treatment that alters mucus layer integrity. Moreover, a mutant that continuously secretes flagellin (LF82-Δflg...Continue Reading

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Citations

Jan 16, 2019·Current Protocols in Mouse Biology·Máximo E LangeG Douglas Inglis
Nov 30, 2018·Inflammatory Bowel Diseases·Alexander M ObercBrian K Coombes
Aug 22, 2020·Médecine sciences : M/S·Sabrine Naimi, Benoit Chassaing
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Jan 31, 2020·Frontiers in Immunology·Emmanuel MontassierLaureline Berthelot
May 30, 2020·International Journal of Molecular Sciences·Mélissa ChervyJérémy Denizot
Jan 1, 2021·Gut Microbes·Yijie GuoNobuhiko Kamada
Mar 28, 2021·Mucosal Immunology·Noëmie DanielBenoit Chassaing
Feb 13, 2020·Current Protein & Peptide Science·Ke ZhangXi Ma

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Methods Mentioned

BETA
IECs
confocal microscopy
IEC
PCR
ELISA

Software Mentioned

Graph Pad Prism
Image Lab
Zen 2011

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