Adaptation of aerobically growing Pseudomonas aeruginosa to copper starvation.

Journal of Bacteriology
Emanuela FrangipaniDieter Haas

Abstract

Restricted bioavailability of copper in certain environments can interfere with cellular respiration because copper is an essential cofactor of most terminal oxidases. The global response of the metabolically versatile bacterium and opportunistic pathogen Pseudomonas aeruginosa to copper limitation was assessed under aerobic conditions. Expression of cioAB (encoding an alternative, copper-independent, cyanide-resistant ubiquinol oxidase) was upregulated, whereas numerous iron uptake functions (including the siderophores pyoverdine and pyochelin) were expressed at reduced levels, presumably reflecting a lower demand for iron by respiratory enzymes. Wild-type P. aeruginosa was able to grow aerobically in a defined glucose medium depleted of copper, whereas a cioAB mutant did not grow. Thus, P. aeruginosa relies on the CioAB enzyme to cope with severe copper deprivation. A quadruple cyo cco1 cco2 cox mutant, which was deleted for all known heme-copper terminal oxidases of P. aeruginosa, grew aerobically, albeit more slowly than did the wild type, indicating that the CioAB enzyme is capable of energy conservation. However, the expression of a cioA'-'lacZ fusion was less dependent on the copper status in the quadruple mutant than in...Continue Reading

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Citations

Mar 18, 2010·Journal of Bacteriology·Shashi ChillappagariMarcus Miethke
Aug 27, 2009·Human Genomics·Deepak Balasubramanian, Kalai Mathee
Jun 26, 2010·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Angélica Reyes-JaraMauricio González
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Mar 7, 2014·Environmental Microbiology Reports·Emanuela FrangipaniDieter Haas
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Aug 8, 2009·FEMS Microbiology Letters·Emanuela Frangipani, Dieter Haas
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Sep 26, 2020·Frontiers in Microbiology·Tania HenríquezHeinrich Jung
Nov 12, 2021·PLoS Biology·Satya Prathyusha BhamidimarriBert van den Berg

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