PMID: 6020481Mar 24, 1967Paper

Adaptation of an insect cell line (Grace's Antheraea cells) to medium free of insect hemolymph

Science
C E YunkerJ Cory

Abstract

Cultures of the insect cell line derived by Grace from Antheraea eucalypti Scott [Austrocaligula eucalypti (Scott)] were successfully adapted to medium supplemented with fetal bovine serum, whole-egg ultrafiltrate, and bovine plasma albumin instead of insect hemolymph. Cells, now in their 37th passage, have a population doubling time of 2.5 days; those of unadapted cultures, 4.2 days.

Citations

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