Adaptation of chimeric retroviruses in vitro and in vivo: isolation of avian retroviral vectors with extended host range

Journal of Virology
E V BarsovStephen H Hughes

Abstract

We have designed and characterized two new replication-competent avian sarcoma/leukosis virus-based retroviral vectors with amphotropic and ecotropic host ranges. The amphotropic vector RCASBP-M2C(797-8), was obtained by passaging the chimeric retroviral vector RCASBP-M2C(4070A) (6) in chicken embryos. The ecotropic vector, RCASBP(Eco), was created by replacing the env-coding region in the retroviral vector RCASBP(A) with the env region from an ecotropic murine leukemia virus. It replicates efficiently in avian DFJ8 cells that express murine ecotropic receptor. For both vectors, permanent cell lines that produce viral stocks with titers of about 5 x 10(6) CFU/ml on mammalian cells can be easily established by passaging transfected avian cells. Some chimeric viruses, for example, RCASBP(Eco), replicate efficiently without modifications. For those chimeric viruses that do require modification, adaptation by passage in vitro or in vivo is a general strategy. This strategy has been used to prepare vectors with altered host range and could potentially be used to develop vectors that would be useful for targeted gene delivery.

References

Apr 1, 1978·Journal of the National Cancer Institute·J A OttenA Brown
Apr 1, 1978·The Journal of Experimental Medicine·C D Scher
Mar 15, 1979·International Journal of Cancer. Journal International Du Cancer·R MandevilleM A Wainberg
May 15, 1975·International Journal of Cancer. Journal International Du Cancer·H OkabeM Hatanaka
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Le Guern, J A Levy
May 1, 1990·Journal of Virology·D H SpectorS A Spector
Jun 1, 1990·AIDS Research and Human Retroviruses·M J MulliganR W Compans
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J PriceC Cepko
Feb 29, 1988·Biochemical and Biophysical Research Communications·M A EglitisW F Anderson
Feb 1, 1973·Journal of the National Cancer Institute·D V AblashiW Turner
Apr 1, 1973·Virology·F L Graham, A J van der Eb
Aug 1, 1993·AIDS Research and Human Retroviruses·A G Laurent-CrawfordA G Hovanessian
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·D G MillerA D Miller
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·M van ZeijlB O'Hara

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Citations

Dec 9, 2010·Nucleic Acids Research·Taco G UilRob C Hoeben
Jul 29, 2015·Proceedings of the National Academy of Sciences of the United States of America·Changming YuMark J Federspiel
Mar 11, 2015·International Journal of Cancer. Journal International Du Cancer·Shripad JoshiHelmut Schaider
Sep 21, 2018·Frontiers in Immunology·Jon Ander Nieto-GaraiHans-Georg Kräusslich

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