Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells

Journal of Microbiological Methods
Michelle J Henry-StanleyCarol L Wells

Abstract

The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures.

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Citations

Aug 7, 2012·Journal of Industrial Microbiology & Biotechnology·Leo L ChanAlnoor Pirani
Apr 6, 2013·Journal of Industrial Microbiology & Biotechnology·Daniel J LavertyLeo Li-Ying Chan
Mar 3, 2010·Antimicrobial Agents and Chemotherapy·Govindsamy VediyappanChristophe d'Enfert
Sep 4, 2007·Applied and Environmental Microbiology·Ekaterina ParamonovaPrashant K Sharma
Feb 9, 2008·The Journal of Clinical Investigation·Guowu HuPeter R Williamson
Jan 18, 2014·Fungal Biology·Whitney M HoldenLouise A Rollins-Smith
Aug 19, 2014·The Journal of Surgical Research·Anthony P CardileKevin S Akers
Jan 30, 2013·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Sofia Costa-de-OliveiraCidália Pina-Vaz
Dec 1, 2010·Mycobiology·Jeong Ah Lee, Hee Youn Chee
Oct 23, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·A Sheila Holmes-SmithGraham Hungerford
Mar 3, 2017·Journal of Fish Diseases·S E Ali, I Skaar
Jul 12, 2005·Yeast
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Barbara BalestrieriJonathan P Arm
May 27, 2016·Science Translational Medicine·Deepak Kumar Vijaya KumarRobert D Moir

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