PMID: 6981579Jun 1, 1982Paper

Adaptation of human long-term B lymphoblastoid cell lines to chemically defined, serum-free media

In Vitro
H MuzikL Bryant

Abstract

Attempts were made to adapt human long-term B lymphoblastoid cell lines to prolonged growth in serum-free, chemically defined media. A newly described medium, which is an enriched modification of Dulbecco's modified Eagle's medium containing additional amino acids and vitamins, was used. The serum is totally replaced by albumin, transferrin, and soybean lipid. The cell lines were all adaptable from RPMI 1640 over a period of time during which the 10% fetal bovine serum (FBS) concentration was reduced and then eliminated in successive steps. After 3 to 6 wk minor alterations in cell shape and adhesion were noted without significant histological changes. Growth characteristics were comparable in the new medium provided a double initial inoculum was used. A panel of cell surface markers, including surface immunoglobulins, Ia antigens, Fc and complement receptors, and T and B erythrocyte rosettes, all showed no altered expression. Molecular genotyping of Ia antigens was carried out by 3-D gel electrophoresis. The antigens showed their full polymorphism without change and were shed into the new culture medium without alteration. Chromosome analysis was performed on Q-banded karyotypes from one of the lines and showed no alteration r...Continue Reading

References

Jan 1, 1979·Canadian Journal of Biochemistry·A K SullivanC Sylvester
Jul 1, 1979·In Vitro·R HolmesN Mohamed
Sep 1, 1977·In Vitro·W G Hamilton, R G Ham
Jan 1, 1980·Cell Biology International Reports·T MaciagR Forand
Mar 1, 1980·Analytical Biochemistry·D Barnes, G Sato
Dec 1, 1980·Cell·D Barnes, G Sato

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Citations

Jan 1, 1992·Pharmacology & Therapeutics·U Bjare
Jan 2, 1992·International Journal of Cancer. Journal International Du Cancer·F ObergK Nilsson
Oct 5, 1988·Biotechnology and Bioengineering·M C GlassyP C Chau

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