Adaptation of rabbit cortical collecting duct HCO3- transport to metabolic acidosis in vitro

The Journal of Clinical Investigation
Shuichi Tsuruoka, G J Schwartz

Abstract

Net HCO3- transport in the rabbit kidney cortical collecting duct (CCD) is mediated by simultaneous H+ secretion and HCO3- secretion, most likely occurring in a alpha- and beta-intercalated cells (ICs), respectively. The polarity of net HCO3- transport is shifted from secretion to absorption after metabolic acidosis or acid incubation of the CCD. We investigated this adaptation by measuring net HCO3- flux before and after incubating CCDs 1 h at pH 6.8 followed by 2 h at pH 7.4. Acid incubation always reversed HCO3- flux from net secretion to absorption, whereas incubation for 3 h at pH 7.4 did not. Inhibition of alpha-IC function (bath CL- removal or DIDS, luminal bafilomycin) stimulated net HCO3- secretion by approximately 2 pmol/min per mm before acid incubation, whereas after incubation these agents inhibited net HCO3- absorption by approximately 5 pmol/min per mm. Inhibition of beta-IC function (luminal Cl- removal) inhibited HCO3- secretion by approximately 9 pmol/min per mm before incubation, whereas after incubation HCO3- absorption by only 3 pmol/min per mm. After acid incubation, luminal SCH28080 inhibited HCO3- absorption by only 5-15% vs the circa 90% inhibitory effect of bafilomycin. In outer CCDs, which contain few...Continue Reading

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Citations

Dec 23, 2006·Histochemistry and Cell Biology·Philipp WehrliJohannes Loffing
Aug 10, 2001·Current Opinion in Nephrology and Hypertension·M Soleimani
Jun 3, 2005·Current Opinion in Nephrology and Hypertension·George J Schwartz, Qais Al-Awqati
Sep 5, 2002·Annual Review of Physiology·Qais Al-Awqati
Jan 10, 2002·The Journal of Clinical Investigation·George J SchwartzQais Al-Awqati
Oct 1, 2003·Biological Chemistry·Qais Al-AwqatiJiro Takito
Oct 6, 2006·Kidney International·D E Wesson
Sep 14, 2000·The Journal of Laboratory and Clinical Medicine·S NakamuraJ H Galla
Jun 27, 2018·Clinical Science·Søren Brandt PoulsenTimo Rieg
Sep 1, 2005·American Journal of Physiology. Renal Physiology·Andrew L SchwadererGeorge J Schwartz
May 18, 2006·American Journal of Physiology. Renal Physiology·Shuichi TsuruokaGeorge J Schwartz
Dec 22, 2006·American Journal of Physiology. Renal Physiology·Sophie de SeigneuxSebastian Frische
Mar 11, 2003·The Journal of Pharmacology and Experimental Therapeutics·Shuichi TsuruokaAkio Fujimura
Jun 11, 1999·The American Journal of Physiology·S NakamuraM Soleimani
Feb 9, 2000·American Journal of Physiology. Renal Physiology·A E FrankI D Weiner
Dec 12, 2001·American Journal of Physiology. Renal Physiology·Snezana PetrovicManoocher Soleimani
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Sep 9, 2004·American Journal of Physiology. Renal Physiology·Seiji WatanabeGeorge J Schwartz
Jun 19, 2015·American Journal of Physiology. Renal Physiology·Jeffrey M PurkersonGeorge J Schwartz
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Mar 22, 2013·American Journal of Physiology. Renal Physiology·Vladimir PechSusan M Wall
Dec 9, 1998·The American Journal of Physiology·S Tsuruoka, G J Schwartz
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Jul 28, 2017·American Journal of Physiology. Renal Physiology·Hu PengGeorge J Schwartz
Feb 12, 1998·The American Journal of Physiology·S Tsuruoka, G J Schwartz
Oct 12, 1999·The American Journal of Physiology·S Tsuruoka, G J Schwartz
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Feb 1, 1997·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·R J Alpern, K Sakhaee

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