PMID: 3706710Mar 1, 1986Paper

Adaptation of the bicinchoninic acid protein assay for use with microtiter plates and sucrose gradient fractions

Analytical Biochemistry
M G Redinbaugh, R B Turley


The bincinchoninic acid protein assay has been adapted for use with microtiter plates and a plate reader to reduce the time needed for analyses. The total volume of the assay was reduced to 0.21 ml with various ratios of sample to reagent volume being used. The assay is sensitive to a limit of less than 1 microgram of bovine serum albumin. The assay is insensitive to the presence of the detergents sodium dodecyl sulfate, Triton X-100, and deoxycholate. In addition, the method has been adapted for determining the protein concentrations of sucrose density gradient fractions, which eliminates the need for removing the sucrose prior to assay.


May 1, 1977·Analytical Biochemistry·J J Sedmak, S E Grossberg
Jan 1, 1976·Analytical Biochemistry·A Bensadoun, D Weinstein
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
May 15, 1985·Analytical Biochemistry·M G Redinbaugh, W H Campbell

❮ Previous
Next ❯


Jul 10, 1999·International Journal of Cancer. Journal International Du Cancer·T IidaT Kondo
Mar 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R J MajeskaT A Einhorn
Aug 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T A EinhornM C Horowitz
Mar 1, 1993·Journal of Biochemical Toxicology·T P RyanS D Aust
Feb 1, 1992·Fish Physiology and Biochemistry·T Gjøen, T Berg
Oct 24, 2012·Journal of Bioenergetics and Biomembranes·Nathan R PerronBärbel Rohrer
Apr 13, 2011·Genes & Nutrition·Franziska Kramer BirkvedIlona Kryspin Sørensen
Jan 16, 1990·Biochimica Et Biophysica Acta·B J CampbellW L Zahler
Jul 17, 1990·European Journal of Pharmacology·A K Mehta, M K Ticku
Oct 15, 1993·Journal of Immunological Methods·D J GillottR T Oliver
May 1, 1990·Journal of Steroid Biochemistry·S A Khan, B C Moulton
Apr 1, 1990·Prostaglandins·R S SpragueA J Lonigro
Feb 1, 1994·Pharmacology, Biochemistry, and Behavior·A K Mehta, M K Ticku
Jul 15, 1994·Brain Research. Developmental Brain Research·X J Hu, M K Ticku
Sep 1, 1994·Neurobiology of Aging·D Linnemann, T Skarsfelt
Feb 1, 1993·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·D LinnemannE Bock
Dec 31, 2003·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·John NovakMadeleine A Kane
Jul 31, 2002·Biochemical and Biophysical Research Communications·Yoshiyuki MiyaharaTakahito Kondo
Nov 30, 1988·Biochemical and Biophysical Research Communications·C A McDevittJ T Andrish
Feb 4, 1998·Chemico-biological Interactions·T MiuraT Ogiso
Sep 25, 2002·Life Sciences·Toshiaki MiuraYukio Fujimoto

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.