Adaptation of the disector method to rare small organelles in TEM sections exemplified by counting synaptic bodies in the rat pineal gland

Journal of Anatomy
Holger JastrowL Vollrath

Abstract

The disector is the only objective method for quantifying particles of variable size in a given volume. With this method, cell organelles are identified on adjacent sections, but only those present in one section are counted. When counting extremely rare structures in transmission electron microscope sections (physical disector), the usual procedure of counting on electron micrographs is limited for economic reasons (e.g. micrographs highly outnumbering the investigated structures). Hence, to apply this unbiased stereological method, a modification of the physical disector concerning 3 aspects has been developed. (1) The prerequisite of screening large corresponding tissue areas (here approximately 65000 microm2) was fulfilled by examining tissue areas along the edges of ultrathin sections. (2) The size of the counting frame was determined by measuring the lengths of the section margins (minus a guard area) by means of a Morphomat. This value was multiplied by the width of the investigated tissue zone, corresponding to the diameter of the electron microscope viewing screen. (3) Disector counting was carried out simultaneously on both sections (bidirectional disector) to improve efficiency. In the present study tiny synaptic bod...Continue Reading

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Citations

Aug 2, 2002·Neuroreport·Christopher P TurnerScott A Rivkees
Jul 4, 2007·Journal of Structural Biology·Dimitri VanheckeMatthias Ochs
Jul 10, 2007·Anatomia, histologia, embryologia·M Ferreira-MedeirosE M Correa-Gillieron
Mar 6, 2012·Anatomia, histologia, embryologia·H ErbagciE Gumusburun
Sep 19, 2006·Micron : the International Research and Review Journal for Microscopy·Holger Jastrow, Jörg Racke

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