Adaptation of wild-type measles virus to CD46 receptor usage

Archives of Virology
L NielsenG Bolt

Abstract

Vaccine strains of measles virus (MV) use CD46 as receptor and downregulate CD46 from the surface of infected cells. MVs isolated and passaged on B-lymphoid cells (wild-type MVs) seem to use another receptor and do not downregulate CD46. In the present study, we found that isolation of MV on human or marmoset B-lymphoid cells did not alter the MV haemagglutinin (H) protein relative to that in the patient. The wild-type isolates were adapted to the human epithelial HEp-2 cell line or the monkey fibroblast Vero cell line. All HEp-2 cell adapted viruses and 1 out of 4 Vero cell adapted viruses acquired the capacity to use CD46 as receptor, as measured by their ability to infect murine cells expressing human CD46. Adaptation to CD46 receptor usage was coupled to substitution of amino acid 481 of the MV H protein from asparagine to tyrosine but not to CD46 downregulation. The present study demonstrates that CD46 receptor usage can be induced by adaptation of wild-type MV to cells that do not express a wild-type receptor and suggests that a similar mechanism acted on the progenitor viruses of the present MV vaccine strains during their isolation and attenuation.

Citations

Jun 17, 2011·The Journal of Infectious Diseases·Bettina BankampPaul A Rota
Jul 5, 2002·Journal of Virology·Urs SchneiderRoberto Cattaneo
Aug 7, 2002·Journal of Virology·Karen BiebackSibylle Schneider-Schaulies
Oct 14, 2006·Uirusu·Shinji Ohno, Yusuke Yanagi
Apr 19, 2002·The Journal of General Virology·Gert BoltMerete Blixenkrone-Møller
Nov 3, 2004·Virology·Elizabeth M HadacStephen J Russell
Aug 24, 2017·Viruses·Daniel N SantiagoHeiko Enderling
Jun 10, 2003·Journal of Medical Virology·Jianhui ZhouTetsuo Nakayama
Mar 3, 2017·Journal of Virology·Sebastien DelpeutChristopher D Richardson
May 12, 2004·The Veterinary Record·A S HammerM Blixenkrone-Moeller
Apr 15, 2003·The Journal of General Virology·Oliver AndresJürgen Schneider-Schaulies
Nov 23, 2010·Expert Review of Vaccines·Patrycja J Lech, Stephen J Russell
Sep 12, 2006·The Journal of General Virology·Yusuke YanagiShinji Ohno

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.