PMID: 6831867Jan 1, 1983

Adaptative features of ectothermic enzymes--IV. Studies on malate dehydrogenase of Astyanax fasciatus (Characidae) from Lobo Reservoir (Såo Carlos, Såo Paulo, Brasil)

Comparative Biochemistry and Physiology. B, Comparative Biochemistry
P H De LucaA R Schwantes

Abstract

1. Skeletal muscle and heart supernatant malate dehydrogenase (s-MDH) from a subtropical fish, Astyanax fasciatus consists of three electrophoretically anodal bands. Each band is a dimer (AA, AB and BB) and two loci are active. 2. In A. fasciatus tissue extracts, A and B subunits are present at differing quantitative levels and their activities are almost season-independent. However, the relative activity of each homodimer in relation to total s-MDH estimated by densitometry of gels or of each homodimer purified by chromatography varies with temperature. The more anodic homodimer is thermolabile and the less anodic one is thermostable. 3. The pH optimum of s-MDH is 7.5, of AA is 6.5 and of BB is 7.8. 4. The BB isozyme is more sensitive to high concentrations of substrate and has a Km temperature-independent. The AA isozyme is not inhibited by high concentrations of oxaloacetate and shows a Km temperature-dependent with a fourteenfold increase between 20 degrees and 40 degrees C.

References

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Citations

Jan 1, 1982·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Z Coppes de AchavalA L Val
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Z L Coppes
Jan 1, 1986·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·E MansiniL P Ribeiro
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·Z L CoppesA R Schwantes
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·A GelmanU Cogan
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·F Basaglia
Dec 31, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G Tripathi, Priyanka Verma
Mar 3, 2004·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Anand S SrivastavaEwa Carrier
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Sep 12, 2019·Anais Da Academia Brasileira De Ciências·Eliane S Rapchan, Walter A Neves

Related Concepts

Acclimatization
Electrophoresis
Hydrogen-Ion Concentration
Alloenzymes
Malate Dehydrogenase
Muscle
Myocardium

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