Adapting chromophore-assisted laser inactivation for high throughput functional proteomics

Briefings in Functional Genomics & Proteomics
Brenda K EustaceD G Jay

Abstract

Recent advances in genomics and proteomics have generated a change in emphasis from hypothesis-based to discovery-based investigations. Genomic and proteomic studies based on differential expression microarrays or comparative proteomics often provide many potential candidates for functionally important roles in normal and diseased cells. High throughput technologies to address protein and gene function in situ are still necessary to exploit these emerging advances in gene and protein discovery in order to validate these identified targets. The pharmaceutical industry is particularly interested in target validation, and has identified it as the critical early step in drug discovery. An especially powerful approach to target validation is a direct protein knockdown strategy called chromophore-assisted laser inactivation (CALI) which is a means of testing the role of specific proteins in particular cellular processes. Recent developments in CALI allow for its high throughput application to address many proteins in tandem. Thus, CALI may have applications for high throughput hypothesis testing, target validation or proteome-wide screening.

Citations

Dec 31, 2014·Bioconjugate Chemistry·Shinichi SatoHiroyuki Nakamura
Aug 22, 2012·Chembiochem : a European Journal of Chemical Biology·Xiaodan LiuThomas J Kodadek
Jul 29, 2010·Physiological Reviews·Dmitriy M ChudakovKonstantin A Lukyanov

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