Adapting the medaka embryo assay to a high-throughput approach for developmental toxicity testing

Neurotoxicology
Sharon L OxendineStephanie Padilla

Abstract

Chemical exposure during embryonic development may cause persistent effects, yet developmental toxicity data exist for very few chemicals. Current testing procedures are time consuming and costly, underlining the need for rapid and low cost screening strategies. While in vitro methods are useful for screening, these methods do not replicate all the intricacies of embryonic development and should ideally be complemented by an in vivo screening strategy. In this study, we modify a medaka fish embryo assay to meet the requirements of high-throughput, developmental toxicant testing in vivo. The Japanese medaka (Oryzias latipes) offers several advantages over traditional mammalian model systems, including economic husbandry, high fecundity, and rapid ex utero development. In most studies where fish eggs are exposed to a chemical, the exposure takes place in a common vessel, with many embryos being exposed to the same solution. This type of design is not amenable to high-throughput methodology, does not allow the investigator to follow the same embryo throughout gestation, and may confound statistical analysis of the results. Therefore, we developed a 96-well microtiter plate method to facilitate exposure of individual medaka embryos...Continue Reading

References

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Citations

Feb 11, 2014·Aquatic Toxicology·Miguel González-DoncelGregoria Carbonell
Jul 3, 2013·BMC Biotechnology·Gerlinde JungThomas Czerny
Dec 21, 2011·Reproductive Toxicology·S PadillaD M Reif
Jun 15, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Napo K M CheungDoris W T Au
Jan 20, 2009·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·David E HintonJeffrey A Yoder
Oct 28, 2008·Neurotoxicology·R C MacPhailS Padilla
Apr 25, 2008·Marine Pollution Bulletin·Michael W CarneySeth W Kullman
Nov 28, 2006·Aquatic Toxicology·Sharon L OxendineStephanie Padilla
Oct 14, 2008·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Adam DavisEdmund J Stellwag
Feb 14, 2013·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·M H HaronA K Dasmahapatra
Jul 2, 2009·Toxicological Sciences : an Official Journal of the Society of Toxicology·Steven O SimmonsRam Ramabhadran
Mar 6, 2019·PloS One·Omar T HammoudaThomas Thumberger
May 19, 2019·G3 : Genes - Genomes - Genetics·Barbara KlotzManfred Schartl
Feb 1, 2009·Current Protocols in Toxicology·Stephanie PadillaDoris W T Au
Mar 4, 2021·Environmental Science & Technology·Donald Wlodkowic, Olivia Campana
May 19, 2009·Reproductive Toxicology·Ingrid W T SelderslaghsHilda E Witters
Jul 1, 2019·G3 : Genes - Genomes - Genetics·Barbara KlotzManfred Schartl
Sep 22, 2009·Environmental Science & Technology·Mark R WiesnerEmily S Bernhardt
Dec 26, 2016·Computers in Biology and Medicine·Elodie PuybareauHugues Talbot

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