Adapting the stretched sample method from tissue profiling to imaging.

Proteomics
Tyler A ZimmermanJonathan V Sweedler

Abstract

The characterization and localization of peptides and proteins in tissues provides information that aids in understanding their function and in characterizing disease states. Over the past decades, the use of MS for the profiling and imaging of biological compounds from tissues has evolved into a powerful modality to accomplish these studies. One recently described sampling approach, the stretched sample method (Monroe, E. B. et al.., Anal. Chem. 2006, 78, 6826-6832), places a tissue section onto an array of glass beads embedded on a Parafilm M membrane. When the membrane is stretched, it separates the tissue section into thousands of cell-sized pieces for tissue profiling by MALDI-MS. The physical separation between beads eliminates analyte redistribution during matrix application and allows long analyte extraction periods without loss of spatial resolution. Here, we enhance this sampling approach by introducing algorithms that enable the reconstruction of ion images from these stretched samples. As the first step, a sample-tailored data acquisition method is devised to obtain mass spectra exclusively from the beads, thereby reducing the time, instrument resources, and data handling required for such MS imaging (MSI) experimen...Continue Reading

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Citations

Oct 29, 2010·Histochemistry and Cell Biology·Jaroslav PólMichael Volný
Apr 8, 2011·Journal of the American Society for Mass Spectrometry·Tyler A ZimmermanJonathan V Sweedler
Oct 25, 2011·Analytical Chemistry·Kevin R TuckerJonathan V Sweedler
Jan 15, 2013·The Analyst·David Gode, Dietrich A Volmer
Dec 15, 2010·Molecular & Cellular Proteomics : MCP·Mélanie LagarrigueCharles Pineau
Oct 20, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hui YeLingjun Li
Oct 18, 2011·Expert Review of Proteomics·Kristin J BoggioJeffrey N Agar
Apr 11, 2013·Advanced Drug Delivery Reviews·Eric M Weaver, Amanda B Hummon
Mar 13, 2010·Journal of Chromatography. a·Erika R Amstalden van HoveRon M A Heeren
Feb 27, 2010·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Angus C GreyKevin L Schey
Jan 13, 2021·Mass Spectrometry Reviews·Carla HarkinDiego F Cobice

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