Adaptive divergence in experimental populations of Pseudomonas fluorescens. II. Role of the GGDEF regulator WspR in evolution and development of the wrinkly spreader phenotype.

Genetics
Patrick GoymerPaul B Rainey

Abstract

Wrinkly spreader (WS) genotypes evolve repeatedly in model Pseudomonas populations undergoing adaptive radiation. Previous work identified genes contributing to the evolutionary success of WS. Here we scrutinize the GGDEF response regulator protein WspR and show that it is both necessary and sufficient for WS. Activation of WspR occurs by phosphorylation and different levels of activation generate phenotypic differences among WS genotypes. Five alleles of wspR, each encoding a protein with a single amino acid substitution, were generated by mutagenesis. Two alleles are constitutively active and cause the ancestral genotype to develop a WS phenotype; the phenotypic effects are allele specific and independent of phosphorylation. Three alleles contain changes in the GGDEF domain and when overexpressed in WS cause reversion to the ancestral phenotype. Ability to mimic this effect by overexpression of a liberated N-terminal domain shows that in WS, regulatory components upstream of WspR are overactive. To connect changes at the nucleotide level with fitness, the effects of variant alleles were examined in both structured and unstructured environments: alleles had adaptive and deleterious effects with trade-offs evident across enviro...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·D H Figurski, D R Helinski
Aug 27, 1996·Biochemistry·I BaikalovR E Dickerson
Feb 1, 1996·Molecular Microbiology·P B Rainey, M J Bailey
Apr 3, 1997·Nature·J DoebleyL Hubbard
Dec 31, 1997·Genetics·J J BullI J Molineux
Jul 17, 1998·Nature·P B Rainey, M Travisano
Mar 2, 1999·Journal of Structural Biology·S Djordjevic, A M Stock
Aug 31, 2000·Annual Review of Biochemistry·A M StockP N Goudreau
Dec 19, 2000·Proteins·J Pei, N V Grishin
Feb 24, 2001·Environmental Microbiology·P B Rainey
Mar 30, 2001·The Journal of Biological Chemistry·S Y LeeD E Wemmer
Sep 15, 2001·FEMS Microbiology Letters·M Y GalperinE V Koonin
Sep 28, 2002·Nature Structural Biology·Ann E MarisRichard E Dickerson
Oct 17, 2002·Protein Science : a Publication of the Protein Society·Sandra Da ReJeffry B Stock
Nov 12, 2002·Journal of Bacteriology·David A D'ArgenioEverett C Pesci
Jan 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Tim F CooperRichard E Lenski
Mar 8, 2003·Molecular Microbiology·Phillip AldridgeUrs Jenal
Jul 3, 2003·Journal of Bacteriology·Victoria L RobinsonAnn M Stock
Sep 5, 2003·Nature·Paul B Rainey, Katrina Rainey
Jan 21, 2004·Molecular Microbiology·Jenny G SmithRobert B Bourret
May 15, 2004·Environmental Microbiology·Michael Y Galperin
Dec 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Carmen ChanTilman Schirmer
Feb 18, 2005·Nature Reviews. Genetics·H Allen Orr
Jul 28, 2005·Molecular Microbiology·Ute RömlingMichael Y Galperin
Sep 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jason W HickmanCaroline S Harwood
Jun 1, 1994·Applied and Environmental Microbiology·J E Loper, S E Lindow

❮ Previous
Next ❯

Citations

Jan 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Christine C SpencerMichael Doebeli
Jul 28, 2010·Biology Letters·Michael J McDonaldPaul B Rainey
Jun 18, 2010·Proceedings. Biological Sciences·Justin R MeyerRees Kassen
Sep 22, 2009·Journal of Bacteriology·Manuel MoisiStefan Schild
Mar 9, 2013·Microbiology and Molecular Biology Reviews : MMBR·Ute RömlingMark Gomelsky
Aug 10, 2006·Annual Review of Genetics·Urs Jenal, Jacob Malone
Mar 28, 2008·PLoS Biology·Nabanita DeHolger Sondermann
Dec 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Régis C E FlohrHubertus J E Beaumont
Feb 20, 2014·International Journal of Evolutionary Biology·Andrew J Spiers
Mar 31, 2009·Future Microbiology·Kristina JonasUte Römling
Nov 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Stephen R GiddensPaul B Rainey
Jun 11, 2014·Nature Communications·Susan F BaileyRees Kassen
Nov 7, 2014·Nature·Katrin HammerschmidtPaul B Rainey
Feb 21, 2016·FEMS Microbiology Reviews·Hans P SteenackersJozef Vanderleyden
Sep 30, 2014·Genomics·Vaughn S CooperCrystal N Ellis
Nov 28, 2015·ELife·Thomas GarciaSilvia De Monte
Aug 22, 2009·Journal of Molecular Biology·Nabanita DeHolger Sondermann
May 5, 2009·Research in Microbiology·G RossignolA Merieau
Jul 1, 2009·Microbial Biotechnology·Ana NavazoMarta Martín
May 29, 2009·Evolution; International Journal of Organic Evolution·Quan-Guo ZhangH Charles J Godfray
Sep 11, 2007·Molecular Microbiology·Vincent T LeeStephen Lory
May 20, 2008·Molecular Microbiology·Jason W Hickman, Caroline S Harwood
Oct 4, 2006·Environmental Microbiology·Susanne UdeAndrew J Spiers
Mar 20, 2010·Environmental Microbiology·Matthew L WorkentineRaymond J Turner
Jul 24, 2015·Molecular Plant Pathology·Sebastian PfeilmeierJacob George Malone
Apr 6, 2016·Journal of Bacteriology·Marivic MartinÁkos T Kovács
Nov 6, 2009·Nature·Hubertus J E BeaumontPaul B Rainey

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.