Nov 1, 1976

Adaptive enhancement of amino acid uptake and exodus by thymic lymphocytes: influence of pH

Journal of Cellular Physiology
W A PeckM A Lichtman

Abstract

Entry of certain free amino acids (alpha aminoisobutyric acid (AIB), alanine and proline), but not of leucine into rat thymic lymphocytes increased progressively when the cells were incubated in amino acid deficient medium. Actinomycin D, cycloheximide, or a high concentration of AIB abolished the time-related increase in AIB accumulation, whereas exposure to a high concentration of leucine had no effect. This phenomenon could not be attributed to a progressive alteration in the nature of the incubation medium nor to reduced transinhibition of AIB uptake. The exodus of AIB also increased with time, but to a smaller degree than AIB entry. Initial rates of AIB entry and exodus increased with increases in the pH of the incubation medium over the range 6.5-8.0. The effects of pH on entry and exodus were time-related, increasing progressively oveb nullified the magnified time related increments in AIB transport caused by prolonged incubation at pH 8.0. The influence of a given pH on transport of AIB decreased rapidly when the cells were transferred to medium of another pH, but this tendency diminished the longer the cells were exposed to the initial pH. pH influenced the entry of alanine and proline in the same fashion as that of AI...Continue Reading

Mentioned in this Paper

T-Lymphocyte
Lymphocytes as Percentage of Blood Leukocytes (Lab Test)
Amino Acids, I.V. solution additive
Uptake
Proline
Etiology
Aminoisobutyric Acids
Cycloheximide
Amino Acid [EPC]
Leucine

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