Adaptive evolution and metabolic engineering of a cellobiose- and xylose- negative Corynebacterium glutamicum that co-utilizes cellobiose and xylose

Microbial Cell Factories
Jungseok LeeHan Min Woo

Abstract

An efficient microbial cell factory requires a microorganism that can utilize a broad range of substrates to economically produce value-added chemicals and fuels. The industrially important bacterium Corynebacterium glutamicum has been studied to broaden substrate utilizations for lignocellulose-derived sugars. However, C. glutamicum ATCC 13032 is incapable of PTS-dependent utilization of cellobiose because it has missing genes annotated to β-glucosidases (bG) and cellobiose-specific PTS permease. We have engineered and evolved a cellobiose-negative and xylose-negative C. glutamicum that utilizes cellobiose as sole carbon and co-ferments cellobiose and xylose. NGS-genomic and DNA microarray-transcriptomic analysis revealed the multiple genetic mutations for the evolved cellobiose-utilizing strains. As a result, a consortium of mutated transporters and metabolic and auxiliary proteins was responsible for the efficient cellobiose uptake. Evolved and engineered strains expressing an intracellular bG showed a better rate of growth rate on cellobiose as sole carbon source than did other bG-secreting or bG-displaying C. glutamicum strains under aerobic culture. Our strain was also capable of co-fermenting cellobiose and xylose withou...Continue Reading

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Citations

Apr 28, 2016·World Journal of Microbiology & Biotechnology·Volker F WendischElvira Sgobba
Jul 7, 2019·Journal of Industrial Microbiology & Biotechnology·Shun Che, Yujie Men
Mar 31, 2019·Biotechnology Journal·Roberto G StellaJulia Frunzke
Sep 25, 2020·Applied Microbiology and Biotechnology·Yanfeng LiuLong Liu
Sep 16, 2018·Applied Microbiology and Biotechnology·Nannan ZhaoSuiping Zheng
Aug 3, 2017·Microbial Cell Factories·Ida LauritsenMorten H H Nørholm
Jan 5, 2021·Frontiers in Bioengineering and Biotechnology·Bin ZhangXiao-Yu Wu
May 26, 2017·Bioresource Technology·Vinuselvi ParisuthamJay D Keasling
May 12, 2021·Microbial Cell Factories·Carina PrellVolker F Wendisch

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Methods Mentioned

BETA
PCR

Software Mentioned

CLgenomics
Pipeline
GenePix Pro
tRNA scan - SE
Gene Designer
RNAmmer

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