Adaptive evolution of microalgae Schizochytrium sp. under high salinity stress to alleviate oxidative damage and improve lipid biosynthesis

Bioresource Technology
Xiao-Man SunHe Huang

Abstract

Lipid accumulation of Schizochytrium sp. can be induced by stress condition, but this stress-induction usually reduce cell growth and cause oxidative damage, which can eventually lower the lipid yield. Here, adaptive laboratory evolution (ALE) combined high salinity was performed to enhance the antioxidant system and lipid accumulation. The final strain ALE150, which was obtained after 150 days, showed a maximal cell dry weight (CDW) of 134.5 g/L and lipid yield of 80.14 g/L, representing a 32.7 and 53.31% increase over the starting strain, respectively. Moreover, ALE150 exhibited an overall higher total antioxidant capacity (T-AOC) and lower reactive oxygen species (ROS) levels than the starting strain. Furthermore, the regulatory mechanisms responsible for the improved performance of ALE150 were analyzed by transcriptomic analysis. Genes related to the antioxidant enzymes and central carbon metabolism were up-regulation. Moreover, the metabolic fluxes towards the fatty acid synthase (FAS) and polyketide synthase (PKS) pathways were also changed.

Citations

Feb 9, 2020·Applied and Environmental Microbiology·Guoxing ZhuJianrong Wu
Jun 1, 2020·Applied Microbiology and Biotechnology·KaiYue LiuWenJing Sun
Jul 30, 2020·Current Opinion in Biotechnology·Mingfeng CaoHuimin Zhao
Jul 12, 2021·Biotechnology Advances·Maria MavrommatiGeorge Aggelis
Sep 15, 2021·Applied Biochemistry and Biotechnology·Xuejiao TianJihong Chen

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