Adaptive responses to osmotic stress in kidney-derived cell lines from Scatophagus argus, a euryhaline fish

Gene
Lang GuiJunbin Zhang

Abstract

The euryhaline fish, the spotted scat (Scatophagus argus), is exceptional for its ability to tolerate rapid fluctuations in salinity. To better understand fish osmoregulation and enable more precise analyses of specific features of adaptive responses to the osmotic stress in fish, a S. argus kidney-derived cell line (SK) was developed and subcultured for more than 70 passages. The cells were mostly fibroblast-like, with a normal diploid karyotype (2n=48). A low-osmolarity-adapted SK cell line (SK-la) was induced by growth in a hypotonic solution (150 mOsm). Effects of different osmotic stresses (150, 300 and 450 mOsm) on cell growth, cell morphology, cell volume changes and cell damage in SK, SK-la and CIK (a kidney-derived cell line from freshwater grass carp) cells were studied. These were compared by use of microscopic observation, flow cytometry and a Na-K-ATPase (NKA) assay. SK cells became smaller and grew rapidly in response to hypotonic stress (150 mOsm), and exhibited no visible morphological changes in response to hypertonic stress (450 mOsm). SK-la grew well by moderate hypertonicity (300 mOsm) but depressed in severe hypertonicity (450 mOsm), the number of unhealthy SK-la cells rose as osmolarity increased. In contr...Continue Reading

References

Feb 15, 1992·The Biochemical Journal·P G PetroniniK P Wheeler
Sep 1, 1991·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·M A Linshaw
May 24, 2003·Annals of the New York Academy of Sciences·Juan M CapassoTomas Berl
May 5, 2004·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Isabelle Leguen, Patrick Prunet
Jul 24, 2004·Biotechnology and Applied Biochemistry·Mon-Han WuJulie Varley
Aug 4, 2004·Current Opinion in Chemical Biology·Bruce S EdwardsLarry A Sklar
Feb 8, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Francesca TrischittaCaterina Faggio
Sep 6, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Stamatis VarsamosGuy Charmantier
Nov 10, 2005·Cell Biology International·Sayyed Mohammad Hadi Alavi, Jacky Cosson
Jan 9, 2007·Pflügers Archiv : European journal of physiology·Roberta R Alfieri, Pier Giorgio Petronini
Mar 16, 2007·Experimental Dermatology·Mónika GöncziLászló Csernoch
Oct 27, 2007·Journal of the American College of Nutrition·Florian Lang
Oct 12, 2007·Physiological Reviews·Maurice B BurgNatalia I Dmitrieva
Jun 6, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·David H Evans
Feb 3, 2009·In Vitro Cellular & Developmental Biology. Animal·L E J LeeN C Bols
Oct 13, 2010·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Caterina FaggioFrancesca Trischitta
Dec 24, 2010·American Journal of Physiology. Cell Physiology·Sang Do LeeH Moo Kwon
Sep 8, 2011·Frontiers in Physiology·Morten Buch Engelund, Steffen S Madsen
Oct 29, 2011·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·R J Gonzalez
Jan 10, 2012·The Journal of Biological Chemistry·Carl D BortnerJohn A Cidlowski
Apr 11, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Mayu Inokuchi, Toyoji Kaneko
May 30, 2012·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Gary Laverty, Erik Skadhauge
Aug 10, 2012·Physiology·Dietmar Kültz
Jan 8, 2013·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Maryline BossusCatherine Lorin-Nebel
May 15, 2013·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Natascha Wosnick, Carolina A Freire
May 17, 2014·SpringerPlus·Xiu-Ying YanZao-He Wu
Jun 1, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Pan HuJunbin Zhang

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