Abstract
The aims of the present study were to characterize the subchronic toxicity of chloroform by measuring tissue injury, repair, and distribution of chloroform and to assess the reasons for the development of tolerance to subchronic chloroform toxicity. Male Swiss Webster (SW) mice were given three dose levels of chloroform (150, 225, and 300 mg/kg/day) by gavage in aqueous vehicle for 30 days. Liver and kidney injury were measured by plasma ALT and BUN, respectively, and by histopathology. Tissue regeneration was assessed by (3)H-thymidine incorporation into hepato- and nephro-nuclear DNA and by proliferating cell nuclear antigen staining. In addition, GSH and CYP2E1 in liver and kidney were assessed at selected time points. The levels of chloroform were measured in blood, liver, and kidney during the dosing regimen (1, 7, 14, and 30 days). Kidney injury was evident after 1 day with all three doses and sustained until 7 days followed by complete recovery. Mild to moderate liver injury was observed from 1 to 14 days with all three dose levels followed by gradual decrease. Significantly higher regenerative response was evident in liver and kidney at 7 days, but the response was robust in kidney, preventing progression of injury beyo...Continue Reading
References
Sep 1, 1991·Cancer Letters·A GreenwellR R Maronpot
Apr 1, 1988·Toxicology and Applied Pharmacology·H M Mehendale, J S Klingensmith
Jan 1, 1984·Toxicology and Applied Pharmacology·R V BranchflowerL R Pohl
Apr 16, 1984·Toxicology·J H SmithJ B Hook
Nov 1, 1994·Environmental Health Perspectives·H M Mehendale
Jul 1, 1994·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·M A Pereira
Apr 1, 1993·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·J L LarsonB E Butterworth
Jul 1, 1996·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·M V TemplinD C Wolf
Nov 29, 1996·Cancer Letters·M V TemplinB E Butterworth
Dec 17, 1997·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·P D LillyR A Pegram
Feb 24, 1998·Carcinogenesis·M V TemplinB E Butterworth
May 8, 1998·Toxicology and Applied Pharmacology·R S Mangipudy, H M Mehendale
Dec 23, 1998·Environmental Health Perspectives·M G Soni, H M Mehendale
May 26, 1999·Regulatory Toxicology and Pharmacology : RTP·M G SoniH M Mehendale
Oct 21, 1999·Toxicology and Applied Pharmacology·A A ConstanB E Butterworth
Feb 8, 2003·International Journal of Toxicology·Sathanandam S AnandHarihara M Mehendale
Jul 5, 2003·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sathanandam S AnandHarihara M Mehendale
Feb 1, 1956·The Biochemical Journal·K BURTON
Aug 1, 1956·Experimental Cell Research·J CHAUVEAUC ROUILLER
Sep 19, 2003·Toxicology and Applied Pharmacology·Pallavi B LimayeHarihara M Mehendale
Apr 5, 2005·Toxicologic Pathology·Harihara M Mehendale
Apr 21, 2005·Chemico-biological Interactions·Thomas L HornDavid L McCormick
Jun 9, 2005·Archives of Toxicology·S S AnandH M Mehendale
Dec 1, 1967·The Biochemical Journal·A A Seawright, A E McLean
Nov 1, 2004·Environmental Toxicology and Pharmacology·Sathanandam S AnandHarihara M Mehendale
Citations
Aug 20, 2009·Critical Reviews in Food Science and Nutrition·Alan R BoobisStephen S Olin
Oct 18, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·K M SutherlandL S Van Winkle
Nov 23, 2007·Toxicology and Applied Pharmacology·Cheng FangJun Gu
Feb 3, 2007·Toxicology·Binu K PhilipHarihara M Mehendale
May 1, 2007·Current Protocols in Toxicology·Sachin S DeviHarihara M Mehendale