Adaptor and clathrin exchange at the plasma membrane and trans-Golgi network

Molecular Biology of the Cell
Xufeng WuLois E Greene

Abstract

We previously demonstrated, using fluorescence recovery after photobleaching, that clathrin in clathrin-coated pits at the plasma membrane exchanges with free clathrin in the cytosol, suggesting that clathrin-coated pits are dynamic structures. We now investigated whether clathrin at the trans-Golgi network as well as the clathrin adaptors AP2 and AP1 in clathrin-coated pits at the plasma membrane and trans-Golgi network, respectively, also exchange with free proteins in the cytosol. We found that when the budding of clathrin-coated vesicle is blocked without significantly affecting the structure of clathrin-coated pits, both clathrin and AP2 at the plasma membrane and clathrin and AP1 at the trans-Golgi network exchange rapidly with free proteins in the cytosol. In contrast, when budding of clathrin-coated vesicles was blocked at the plasma membrane or trans-Golgi network by hypertonic sucrose or K(+) depletion, conditions that markedly affect the structure of clathrin-coated pits, clathrin exchange was blocked but AP2 at the plasma membrane and both AP1 and the GGA1 adaptor at the trans-Golgi network continue to rapidly exchange. We conclude that clathrin-coated pits are dynamic structures with rapid exchange of both clathrin...Continue Reading

References

Aug 31, 1992·Biochemical and Biophysical Research Communications·S M VoglmaierA B Theibert
Feb 1, 1989·The Journal of Cell Biology·J E Heuser, R G Anderson
Nov 1, 1994·The Journal of Cell Biology·H DamkeS L Schmid
Nov 1, 1996·The Journal of Cell Biology·A Wilde, F M Brodsky
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A SubtilT E McGraw
Jul 10, 1999·Science·M Marsh, H T McMahon
Nov 13, 1999·Nature Cell Biology·I GaidarovJ H Keen
Feb 9, 2000·Current Biology : CB·H Stenmark
Mar 1, 2000·Trends in Biochemical Sciences·M A McNivenY Yoon
Jun 14, 2000·Current Opinion in Neurobiology·L BrodinO Shupliakov
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·T Kirchhausen
Apr 13, 2001·Cell·R PuertollanoJ S Bonifacino
Jun 16, 2001·Molecular Biology of the Cell·G CostagutaG S Payne
Jul 17, 2001·Current Opinion in Cell Biology·M S Robinson, J S Bonifacino
Jul 17, 2001·Current Opinion in Cell Biology·D Danino, J E Hinshaw
Oct 18, 2001·The Journal of Cell Biology·X WuL E Greene
May 10, 2002·Nature·John F PresleyJennifer Lippincott-Schwartz

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Citations

Jul 28, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Vyas RamananRavi Radhakrishnan
Oct 5, 2007·Molecular Biology of the Cell·Virginie BraunFlorence Niedergang
Apr 25, 2008·Molecular Biology of the Cell·Dong-Won LeeLois E Greene
Mar 3, 2012·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Paula FlorianAnca Roseanu
Oct 12, 2004·Annual Review of Cell and Developmental Biology·David J OwenPhilip R Evans
Jun 2, 2009·Frontiers in Bioscience (Landmark Edition)·Kailash N Pandey
Jun 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lars HinrichsenErnst J Ungewickell
Aug 29, 2014·Molecular Biology of the Cell·Joe GroveMark Marsh
Sep 25, 2012·Traffic·Yi LuoJames H Keen
Sep 8, 2010·Current Opinion in Biotechnology·Kailash N Pandey
Jul 7, 2009·Gene Expression Patterns : GEP·Christian WingenMatthias Behr
Oct 14, 2008·International Journal for Parasitology·Fernando López-SotoMireya de la Garza
Aug 11, 2007·Seminars in Cell & Developmental Biology·Els Wessels, Jeremy C Simpson
Jul 17, 2007·Current Opinion in Cell Biology·Ernst J Ungewickell, Lars Hinrichsen
Oct 22, 2004·The EMBO Journal·Gerrit J K PraefckeHarvey T McMahon
Feb 25, 2006·Traffic·Silvia Vergarajauregui, Rosa Puertollano
Nov 9, 2007·Traffic·Guruprasad R MedigeshiPeter Schu
Jul 2, 2011·Traffic·Wouter K den Otter, Wim J Briels
Feb 18, 2010·Developmental Cell·Margaret S RobinsonSamuel D Foster
Aug 18, 2010·Biophysical Journal·Wouter K den OtterW J Briels
Jun 22, 2016·Current Opinion in Neurobiology·Naoya Yamashita, Rejji Kuruvilla
Jul 23, 2004·Physiological Reviews·Michael G Roth
May 3, 2003·Nature Reviews. Molecular Cell Biology·Juan S Bonifacino, Jennifer Lippincott-Schwartz
Jan 13, 2006·The Journal of Biological Chemistry·Mehul ShahPravin B Sehgal
Aug 8, 2015·The Biochemical Journal·Macarena Rodriguez-WalkerJosé L Daniotti

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