ADCY5 gene expression in adipose tissue is related to obesity in men and mice

PloS One
Anja KniggeMatthias Blüher

Abstract

Genome wide association studies revealed an association of the single nucleotide polymorphism rs11708067 within the ADCY5 gene--encoding adenylate cyclase 5--with increased type 2 diabetes (T2D) risk and higher fasting glucose. However, it remains unclear whether the association between ADCY5 variants and glycemic traits may involve adipose tissue (AT) related mechanisms. We therefore tested the hypothesis that ADCY5 mRNA expression in human and mouse AT is related to obesity, fat distribution, T2D in humans and high fat diet (HFD) in mice. We measured ADCY5 mRNA expression in paired samples of visceral and subcutaneous adipose tissue from 244 individuals with a wide range of body weight and parameters of hyperglycemia, which have been genotyped for rs11708067. In addition, AT ADCY5 mRNA was assessed in C57BL/6NTac which underwent a 10 weeks standard chow (n = 6) or high fat diet (HFD, n = 6). In humans, visceral ADCY5 expression is significantly higher in obese compared to lean individuals. ADCY5 expression correlates with BMI, body fat mass, circulating leptin, fat distribution, waist and hip circumference, but not with fasting plasma glucose and HbA1c. Adcy5 expression in mouse AT is significantly higher after a HFD compared...Continue Reading

References

Sep 1, 2000·American Journal of Physiology. Renal Physiology·N DeferJ Hanoune
Feb 13, 2001·Molecular and Cellular Endocrinology·V VanvoorenJ E Dumont
Dec 28, 2006·Hypertension·M Cecilia Ortiz-CapisanoWilliam H Beierwaltes
Jul 30, 2008·Molecular and Cellular Endocrinology·R Huw Jones, Susan E Ozanne
Sep 8, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Che-Lin HuDorothy E Vatner
Jan 1, 2008·Obesity Facts·Matthias Bluher
Apr 8, 2010·Nature Genetics·Rachel M FreathyMark I McCarthy
Jun 24, 2010·American Journal of Physiology. Endocrinology and Metabolism·Nora KlötingMatthias Blüher
Mar 13, 2012·Current Opinion in Clinical Nutrition and Metabolic Care·Hanieh Yaghootkar, Rachel M Freathy
Jun 5, 2013·Best Practice & Research. Clinical Endocrinology & Metabolism·Matthias Blüher
Jun 14, 2013·European Journal of Endocrinology·Hanna HuopioMarkku Laakso
Apr 18, 2014·Diabetes·David J HodsonGuy A Rutter
Aug 15, 2014·Aging Cell·Timothy P CashManuel Serrano

❮ Previous
Next ❯

Citations

May 1, 2016·Cell and Tissue Research·Outi HuttalaT Ylikomi
May 1, 2021·International Journal of Molecular Sciences·Sebastian DommelMatthias Blüher
Oct 27, 2021·Physiological Reviews·Katrina F OstromRennolds S Ostrom

❮ Previous
Next ❯

Methods Mentioned

BETA
exome sequencing
transgenic
X-ray
Assay
PCR
electrophoresis
Genotyping

Software Mentioned

TaqMan
SPSS
TaqMan Software
ARRIVE

Related Concepts

Related Feeds

Biomarkers for Type 2 Diabetes

Biomarkers can help understand chronic diseases and assist in risk prediction for prevention and early detection of diseases. Here is the latest research on biomarkers in type 2 diabetes, a disease in which the body is unable to produce or properly use insulin.