PMID: 26780407DOI: 10.1038/nchembio.2002Jan 19, 2016Paper
Addicting diverse bacteria to a noncanonical amino acid
Nature Chemical Biology
Drew S TackAndrew D Ellington
Abstract
Engineered orthogonal translation systems have greatly enabled the expansion of the genetic code using noncanonical amino acids (NCAAs). However, the impact of NCAAs on organismal evolution remains unclear, in part because it is difficult to force the adoption of new genetic codes in organisms. By reengineering TEM-1 β-lactamase to be dependent on a NCAA, we maintained bacterial NCAA dependence for hundreds of generations without escape.
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