Adding selectivity to antimicrobial peptides: rational design of a multidomain peptide against Pseudomonas spp

Antimicrobial Agents and Chemotherapy
Randal EckertWenyuan Shi


Currently available antimicrobials exhibit broad killing with regard to bacterial genera and species. Indiscriminate killing of microbes by these conventional antibiotics can disrupt the ecological balance of the indigenous microbial flora, often resulting in negative clinical consequences. Species-specific antimicrobials capable of precisely targeting pathogenic bacteria without damaging benign microorganisms provide a means of avoiding this problem. In this communication, we report the successful creation of the first synthetic, target-specific antimicrobial peptide, G10KHc, via addition of a rationally designed Pseudomonas-specific targeting moiety (KH) to a generally killing peptide (novispirin G10). The resulting chimeric peptide showed enhanced bactericidal activity and faster killing kinetics against Pseudomonas spp. than G10 alone. The enhanced killing activities are due to increased binding and penetration of the outer membrane of Pseudomonas sp. cells. These properties were not observed in tests of untargeted bacterial species, and this specificity allowed G10KHc to selectively eliminate Pseudomonas spp. from mixed cultures. This work lays a foundation for generating target-specific "smart" antimicrobials to complemen...Continue Reading


Mar 6, 1998·Trends in Biotechnology·R E Hancock, R I Lehrer
Dec 22, 1999·Science·Eefjan BreukinkBen de Kruijff
Mar 17, 2000·Immunological Reviews·H G Boman
Apr 5, 2002·Clinical Microbiology Reviews·Jeffrey B LyczakGerald B Pier
May 1, 2002·Respiratory Research·Mark T AlbrechtNeal L Schiller
May 23, 2002·Antimicrobial Agents and Chemotherapy·Lars SteinstraesserStewart C Wang
Dec 20, 2002·Biopolymers·Yechiel Shai
May 22, 2003·Bioconjugate Chemistry·Rainer FischerRoland Brock
Nov 1, 1952·Journal of Bacteriology·N D ZINDER, J LEDERBERG
Nov 7, 2003·International Journal of Antimicrobial Agents·Kim A BrogdenBrian F Tack
Jan 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ki-Jong RheeKatherine L Knight
Feb 1, 1952·Journal of General Microbiology·E S DUTHIE, L L LORENZ
Mar 24, 2004·Nature Reviews. Microbiology·Timothy J Mitchell
May 5, 2004·Best Practice & Research. Clinical Gastroenterology·Karen M PickardThomas T MacDonald
May 7, 2004·Journal of Clinical Gastroenterology·Kyu Y RheeCharles Maltz
May 26, 2004·Immunology Letters·Helena Tlaskalová-HogenováAlena Kokesová
Jul 30, 2004·International Journal of Food Microbiology·Hui LiuLiping Sun
Jul 31, 2004·The Journal of Nutrition·R A Rastall
Dec 2, 2004·Nature Reviews. Immunology·Philippe J Sansonetti
Feb 11, 2005·Nature Reviews. Microbiology·Kim A Brogden
Apr 5, 2005·The Journal of Biological Chemistry·Per Eugen KristiansenJon Nissen-Meyer
Sep 17, 2005·FEMS Microbiology Letters·Fengxia QiSteven D Goodman


Apr 27, 2011·Antimicrobial Agents and Chemotherapy·Christopher W KaplanRandal Eckert
Aug 31, 2006·Antimicrobial Agents and Chemotherapy·Randal EckertWenyuan Shi
Oct 25, 2006·Antimicrobial Agents and Chemotherapy·Randal EckertWenyuan Shi
Oct 27, 2006·Antimicrobial Agents and Chemotherapy·Alberto MuñozJose F Marcos
Feb 14, 2007·Antimicrobial Agents and Chemotherapy·Jian HeWenyuan Shi
Mar 10, 2010·Antimicrobial Agents and Chemotherapy·Jian HeRandal Eckert
Mar 7, 2012·Antimicrobial Agents and Chemotherapy·E N LorenzónE M Cilli
Aug 23, 2011·PloS One·Daniel YarbroughWenyuan Shi
Feb 2, 2013·PloS One·Valeriy DomenyukChris W Diehnelt
Oct 30, 2009·International Journal of Molecular Sciences·Lars SteinstraesserSammy Al-Benna
Feb 9, 2010·Indian Journal of Dental Research : Official Publication of Indian Society for Dental Research·Sonia B BhardwajK Gauba
Jun 24, 2008·Critical Reviews in Microbiology·Raymond Murray Dawson, Chun-Qiang Liu
Mar 19, 2011·Expert Opinion on Therapeutic Patents·Zhong-Cheng Song, Hai-Liang Zhu
Jun 5, 2012·Journal of Bioscience and Bioengineering·Wataru AokiMitsuyoshi Ueda
Nov 5, 2010·Veterinary Immunology and Immunopathology·Mandar PatgaonkarKudumula Venkata Rami Reddy
Jul 28, 2010·Bioorganic & Medicinal Chemistry·Partha Pratim BosePer I Arvidsson
Feb 4, 2009·International Journal of Antimicrobial Agents·Jian HeRandal Eckert
Oct 9, 2007·Advances in Pediatrics·David M Krol, Michael P Nedley
Oct 12, 2007·Chemical Biology & Drug Design·Randal EckertWenyuan Shi
Aug 7, 2012·Chemical Biology & Drug Design·Wataru AokiMitsuyoshi Ueda
May 21, 2013·Journal of Chemotherapy·Carlo L RomanòLorenzo Drago
Aug 21, 2015·Organic & Biomolecular Chemistry·Shah Md Abdur RaufBahareh Honarparvar
Feb 17, 2015·Journal of Peptide Science : an Official Publication of the European Peptide Society·Lanlan YuLingbo Qu
Jun 6, 2015·Probiotics and Antimicrobial Proteins·Gargi Pal, Sheela Srivastava
Apr 25, 2012·Biochimica Et Biophysica Acta·Cristina ChamorroRoland J Pieters
Oct 27, 2006·FEMS Microbiology Letters·Huichun TongXiuzhu Dong
Aug 1, 2014·PLoS Pathogens·Rebecca A FlahertyShaun W Lee
Jul 16, 2013·Journal of Laboratory Automation·Peter B LillehojChih-Ming Ho
Nov 28, 2013·Pharmaceuticals·Wataru Aoki, Mitsuyoshi Ueda
Nov 30, 2013·Pharmaceuticals·Ali Adem Bahar, Dacheng Ren

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