Adding the oxygen carrier M101 to a cold-storage solution could be an alternative to HOPE for liver graft preservation

JHEP Reports : Innovation in Hepatology
Pierre AlixKarim Boudjema

Abstract

Hypothermic oxygenated machine perfusion (HOPE) is a promising technique for providing oxygen to the liver during graft preservation; however, because of associated logistical constraints, addition of an oxygen transporter to static cold-storage solutions (SCS) might be easier. M101 is marine worm haemoglobin that has been shown to improve kidney preservation in the clinic when added to SCS. This study evaluated the effects of the addition of M101 to SCS on the quality of pig liver graft preservation. Pig liver grafts were preserved using SCS, HOPE, or SCS+M101, and the liver functions were compared during cold preservation and after orthotopic allotransplantation (OLT) in pigs. During preservation of the liver grafts, mitochondrial function, ATP synthesis, antioxidant capacities, and hepatocyte architecture were better preserved, and free radical production, antioxidant activities, and inflammatory mediators were lower, with HOPE or SCS+M101 than with SCS alone. However, after 1 h of preservation, liver functions with HOPE were superior to those with SCS+M101. After 6 h of preservation and OLT, blood levels of aspartate and alanine aminotransferases and lactate dehydrogenase increased with a peak effect at Day 1 post-transplan...Continue Reading

References

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Citations

Dec 12, 2020·JHEP Reports : Innovation in Hepatology·Isabel M A BrüggenwirthVincent E de Meijer
Jan 1, 2021·International Journal of Molecular Sciences·Silke B BodewesRobert J Porte
Aug 28, 2021·International Journal of Molecular Sciences·Njikem Asong-FontemRené Adam

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Methods Mentioned

BETA
sedation
electron
biopsies
ELISA
electron microscopy
transmission electron microscopy
MDA

Software Mentioned

HOPE
INRAE
Graph Pad Prism
Graph Pad

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