Addition of cloned beta-glucosidase enhances the degradation of crystalline cellulose by the Clostridium thermocellum cellulose complex

Biochemical and Biophysical Research Communications
S K Kadam, Arnold L Demain

Abstract

A thermostable beta-glucosidase from Clostridium thermocellum which is expressed in Escherichia coli was used to determine the substrate specificity of the enzyme. A restriction map of the beta-glucosidase gene cloned in plasmid pALD7 was determined. Addition of the E. coli cell extract (containing the beta-glucosidase) to the cellulase complex from C. thermocellum increased the conversion of crystalline cellulose (Avicel) to glucose. The increase was specifically due to hydrolysis of the accumulated cellobiose. A cellulose degradation process using beta-glucosidase to assist the potent cellulase complex of C. thermocellum, as shown here can open the way for industrial saccharification of cellulose to glucose.

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Citations

Oct 24, 2009·Bioprocess and Biosystems Engineering·Sang-Mok LeeJung-Heon Lee
Aug 12, 2009·Applied Biochemistry and Biotechnology·Bruno BenolielLidia Maria Pepe de Moraes
Apr 25, 2012·Applied Biochemistry and Biotechnology·S LinggangS Abd-Aziz
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Jun 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Gilad GefenEdward A Bayer
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