PMID: 8961306Nov 15, 1996Paper

Addition of phosphatidylcholine-phospholipase C induces cellular redistribution and phosphorylation of protein kinase C zeta in C 6 glial cells

Neuroscience Letters
I Galve-RoperhI Diaz-Laviada

Abstract

Phosphatidylcholine breakdown has been shown to play a critical role in signal transduction involving generation of a number of second messengers [Exton, J.H., Biochim. Biophys. Acta, 1212 (1994) 26-42]. In the present report we demonstrate by immunofluorescence that short-treatment of C 6 glial cells with phosphatidylcholine-hydrolyzing phospholipase C (PC-PLC), changes the intracellular localization of protein kinase C (PKC) zeta from the cytoplasm to a perinuclear region. Western blot analysis also showed a redistribution of PKC zeta after incubation of cells with PC-PLC. To test whether these changes were accompanied by an activation of the enzyme, we measured the extent of phosphorylation of PKC zeta by immunoprecipitation from 32P-labelled cells. Short-treatment with PC-PLC resulted in enhanced phosphorylation of the higher Mr PKC zeta in C 6 glial cells.

References

May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·Y OnoY Nishizuka
Apr 14, 1994·Biochimica Et Biophysica Acta·J H Exton
Jan 1, 1994·Annual Review of Neuroscience·C Tanaka, Y Nishizuka
Sep 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·T C SacktorE Sublette
Apr 1, 1993·Neuroreport·K KumarB Madhukar

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Citations

Feb 5, 1998·Brain Research. Molecular Brain Research·I Galve-RoperhI Díaz-Laviada

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