PMID: 16505580Mar 1, 2006Paper

Addition of phosphotungstic acid to ethanol for dehydration improves both the ultrastructure and antigenicity of pituitary tissue embedded in LR White acrylic resin

Archives of Histology and Cytology
Yuko SakaiTsuyoshi Watanabe

Abstract

Although hydrophilic acrylic resins including LR White have been widely utilized as embedding media for immunocytochemical use, the constituents of tissues are often extracted by the resin monomer during the infiltration process of the embedment, resulting in a discernible impairment of the ultrastructure when the tissue is weakly fixed only with aldehydes. To minimize the extraction by the resin monomer, the embedding procedure with LR White resin was reexamined in the present study. Among the treatments tested, a partial dehydration with 70% ethanol containing 2% phosphotungstic acid (PTA) well preserved the ultrastructure of the pituitary tissue without spoiling the antigenicity of LHbeta and other representative markers for the Golgi apparatus. In addition, treatment with 1% tannic acid (TA) prior to the dehydration described above synergistically improved both the ultrastructure and antigenicity of the tissue so that the orientation of the Golgi apparatus could be determined by double immunogold labeling with commercially available anti-GM130 and anti-TGN38 antibodies. The ultrathin sections from the LR White-embedded tissue treated with TA and dehydrated in 70% ethanol containing 2% PTA also enhanced contrast without conv...Continue Reading

References

Jun 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M A BerrymanA L Hubbard
Feb 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M A Berryman, R D Rodewald
Mar 1, 1989·The Histochemical Journal·K T Tokuyasu
Jul 1, 1988·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M WatanabeY Uchiyama
Aug 1, 1986·The Histochemical Journal·C A Maldonado, A Aoki
Feb 1, 1987·The Histochemical Journal·G R Newman
May 1, 1973·The Journal of Cell Biology·K T Tokuyasu
Feb 1, 1966·Journal of Ultrastructure Research·D C Pease
Mar 1, 1968·Journal of Ultrastructure Research·F E Bloom, G K Aghajanian
Mar 1, 1969·The Journal of Cell Biology·L Silverman, D Glick
Nov 1, 1971·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J E Scott
Jan 1, 1982·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M Bendayan
Mar 1, 1995·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·K D PhendR J Weinberg
Aug 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·T Takizawa, J M Robinson
Sep 1, 1994·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H Tamaki, S Yamashina
Jan 21, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Yuko SakaiTsuyoshi Watanabe
Sep 7, 1957·Annals of the New York Academy of Sciences·R J KOEGELS M BIRNBAUM

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Citations

Jul 13, 2006·Histochemistry and Cell Biology·Hiroki TeramaeToshihiko Iwanaga
May 9, 2012·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Tsuyoshi WatanabeTatsuo Ushiki
Sep 11, 2007·Archives of Histology and Cytology·Katsuyuki KitaharaTsuyoshi Watanabe
Aug 7, 2010·Human Pathology·Virawudh SoontornniyomkijHarry V Vinters
Nov 15, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Masahiro HosakaTetsuro Izumi
Jul 25, 2020·Current Protocols in Cell Biology·Derek P Narendra, Matthew L Steinhauser

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