Addition of Polybrene improves stability of organophosphate hydrolase immobilized in poly(vinyl alcohol) cryogel carrier

Journal of Biochemical and Biophysical Methods
Elena EfremenkoS D Varfolomeyev


Organophosphate hydrolase, covalently attached to the beads of poly(vinyl alcohol) cryogel in the presence of Polybrene, was fivefold more stable in 15% (v/v) ethanol solution than the free enzyme. Immobilized biocatalyst, prepared with an addition of Polybrene, retained a half of its initial activity in 50% (v/v) aqueous ethanol solution, 90% of activity during 10 working cycles of Paraoxon hydrolysis and 85% of activity after storage in the 50 mM CHES buffer (pH 9.0) at room temperature for 2 months.


Jun 25, 2009·Applied Biochemistry and Biotechnology·Elena EfremenkoBo Mattiasson
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Feb 27, 2015·Journal of Biomaterials Science. Polymer Edition·Deniz Aktaş UygunAdil Denizli

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Alkalescens-Dispar Group
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