Addition of Riboflavin-Coupled Magnetic Beads Increases Current Production in Bioelectrochemical Systems via the Increased Formation of Anode-Biofilms

Frontiers in Microbiology
Tutut ArindaKatrin Sturm-Richter

Abstract

Shewanella oneidensis is one of the best-understood model organisms for extracellular electron transfer. Endogenously produced and exported flavin molecules seem to play an important role in this process and mediate the connection between respiratory enzymes on the cell surface and the insoluble substrate by acting as electron shuttle and cytochrome-bound cofactor. Consequently, the addition of riboflavin to a bioelectrochemical system (BES) containing S. oneidensis cells as biocatalyst leads to a strong current increase. Still, an external application of riboflavin to increase current production in continuously operating BESs does not seem to be applicable due to the constant washout of the soluble flavin compound. In this study, we developed a recyclable electron shuttle to overcome the limitation of mediator addition to BES. Riboflavin was coupled to magnetic beads that can easily be recycled from the medium. The effect on current production and cell distribution in a BES as well as the recovery rate and the stability of the beads was investigated. The addition of synthesized beads leads to a more than twofold higher current production, which was likely caused by increased biofilm production. Moreover, 90% of the flavin-coup...Continue Reading

References

Nov 27, 2002·Antonie van Leeuwenhoek·Kenneth H NealsonBrent McKee
Mar 7, 2003·Applied and Environmental Microbiology·Daniel R Bond, Derek R Lovley
Jun 24, 2005·Nature·Gemma RegueraDerek R Lovley
Jul 20, 2006·Proceedings of the National Academy of Sciences of the United States of America·Yuri A GorbyJim K Fredrickson
Mar 5, 2008·Proceedings of the National Academy of Sciences of the United States of America·Enrico MarsiliDaniel R Bond
Mar 31, 2009·Nature Reviews. Microbiology·Bruce E Logan
Aug 22, 2009·Applied Microbiology and Biotechnology·Sharon B Velasquez-OrtaHarald von Canstein
Aug 9, 2011·Nature Nanotechnology·Nikhil S MalvankarDerek R Lovley
Nov 11, 2011·Applied Microbiology and Biotechnology·Evan D Brutinel, Jeffrey A Gralnick
Apr 5, 2012·Faraday Discussions·Nicholas F PolizziDavid N Beratan
Dec 17, 2014·Frontiers in Microbiology·Liang ShiJohn M Zachara
Dec 25, 2015·Journal of Microbiology and Biotechnology·Kerstin DolchJohannes Gescher
May 3, 2016·Advances in Microbial Physiology·G F WhiteT A Clarke
May 7, 2016·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Dan-Dan ZhaiYang-Chun Yong
Jan 1, 2014·International Scholarly Research Notices·Ronald Bartzatt, Tasloach Wol
Aug 11, 2016·Microbial Biotechnology·Kenneth H Nealson, Annette R Rowe
Jan 7, 2017·Biotechnology and Bioengineering·Thea BursacJohannes Gescher
Oct 27, 2017·Advances in Biochemical Engineering/biotechnology·Francesca SimonteKatrin Sturm-Richter
Jun 23, 2018·Biotechnology & Genetic Engineering Reviews·Muhammad Mohsin JavedSana Zahoor
Jul 12, 2018·Molecular Microbiology·Sebastian BeblawyJohannes Gescher

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Citations

Mar 12, 2021·Biotechnology Advances·Igor VassilevMarika Kokko
Jun 5, 2021·Biotechnology for Biofuels·Miriam EdelJohannes Gescher

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Methods Mentioned

BETA
PCA
PCR
fluorescence microcopy
fluorescence microscopy

Software Mentioned

Leica LAS AF Lite

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