Addition of tetrodotoxin alters the morphology of thalamocortical axons in organotypic cocultures

Journal of Neuroscience Research
M F Wilkemeyer, K J Angelides

Abstract

Living organotypic cocultures of rat thalamic and cortical explants were used to examine the effects of blocking action potential activity on the morphological development of axons in the mammalian neocortex. Studies in vivo have suggested that blocking sodium channel-dependent activity influences the growth characteristics of thalamocortical axons during development. We have extended these observations by using an in vitro system that affords more direct observational analysis of the early events of axonal growth in an accessible cellular environment DiI-labeled thalamocortical axons grow exuberantly into the target cortex and establish axonal connections that reflect the events of early thalamocortical afferent development. Within these cocultures, the morphological features of DiI-labeled axons can be readily distinguished. Tracings of thalamocortical axons were quantitated with respect to number, length, and termination pattern of axonal branches, as well as number of varicosities. Addition of the voltage-dependent sodium channel blocker, tetrodotoxin, to cocultures did not change the general pattern of thalamocortical axonal ingrowth or the average length of collateral branches of these axons. However, in the presence of t...Continue Reading

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Citations

Jul 20, 2005·PLoS Biology·Carlos Portera-CailliauKarel Svoboda
Mar 8, 2006·The European Journal of Neuroscience·David J PriceZoltán Molnár
Jun 20, 2008·Reviews in the Neurosciences·Yoshiaki TagawaTomoo Hirano
Aug 10, 2002·The European Journal of Neuroscience·Gillian Anderson, David J Price

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