PMID: 10960734Aug 29, 2000Paper

Additional phosphate stabilises uninterrupted growth of a Dioscorea deltoidea cell culture

Plant Science : an International Journal of Experimental Plant Biology
O KandarakovR Ehwald


Suspension cells of Dioscorea deltoidea Wall (strain D-1) were maintained in a semicontinuous culture (SCC) in shake flasks at a high growth rate. It was shown that continuous propagation growth of this culture is unstable on Murashige's and Skoog's (MS) medium due to P starvation. On a P-enriched MS-medium the SCC was stable even at mean specific growth rates >0.3 day(-1). Highest volumetric concentrations of furostanol glycosides were obtained, when a P-enriched SCC was not further subcultivated but fed with sucrose. The investigated culture is able to control phosphate uptake and to prevent toxicity on media with excess P. High concentrations of cellular P(i) did not effect the ratio of furostanol to starch.


Dec 1, 1965·The British Journal of Psychiatry : the Journal of Mental Science·A Coppen
Dec 1, 1996·Plant Cell Reports·A Oostdam, L H van der Plas

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