PMID: 9437731Jan 23, 1998Paper

Additional recognition sites in the C-terminal heparin-binding domain of fibronectin promote adhesion of PMA-treated U937 cells

Peptides
K KatoH Mohri

Abstract

Recently we have shown an evidence that a peptide, corresponding to residues Gln1892 to Gly1910, from the C-terminal heparin-binding domain of fibronectin promotes adhesion of phorbol-12-myristate 13-acetate (PMA)-treated U937 cells and binds to both integrin alpha 4 beta 1 and glycosaminoglycans on U937 cells surface. We present additional adhesion-promoting sites to PMA-treated U937 cells present in the C-terminal heparin-binding domain of fibronectin. Three synthetic peptides (residues Ala1819 to Lys1830, designated E5; Thr1828 to Gly1940, E4; and Lys1946 to Leu1963, D1) were active to inhibit adhesion of PMA-treated U937 cells to the 29-kDa fragment comprising the C-terminal heparin-binding domain of fibronectin. Scrambled versions of these peptides had no inhibitory activity on this adhesion. The IgG-conjugated peptides (IgG-E5, IgG-E4, and IgG-D1) were also active and supported adhesion to an extent comparable to that of the 29-kDa fragment. The adhesion of PMA-treated U937 cells on these three IgG-conjugated peptides was only inhibited by glycosaminoglycans and not by integrin alpha 4 beta 1. These results indicate that additional adhesion-promoting sequences are present in the C-terminal heparin-binding domain of fibron...Continue Reading

References

Mar 1, 1988·The Journal of Cell Biology·K R GehlsenE Ruoslahti
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Jan 1, 1985·Annual Review of Cell Biology·R Hynes
Jan 1, 1985·Journal of Cellular Biochemistry·K M Yamada, D W Kennedy
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M D Pierschbacher, E Ruoslahti
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle

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Citations

Mar 7, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ryusuke YoshimiYoshiaki Ishigatsubo

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