Addressing the Compartmentalization of Specific Integrin Heterodimers in Mouse Sperm

International Journal of Molecular Sciences
Michaela FrolikovaKaterina Dvorakova-Hortova

Abstract

Integrins are transmembrane cell receptors involved in two crucial mechanisms for successful fertilization, namely, mammalian intracellular signaling and cell adhesion. Integrins α6β4, α3β1 and α6β1 are three major laminin receptors expressed on the surface of mammalian cells including gametes, and the presence of individual integrin subunits α3, α6, β1 and β4 has been previously detected in mammalian sperm. However, to date, proof of the existence of individual heterodimer pairs in sperm and their detailed localization is missing. The major conclusion of this study is evidence that the β4 integrin subunit is expressed in mouse sperm and that it pairs with subunit α6; additionally, there is a detailed identification of integrin heterodimer pairs across individual membranes in an intact mouse sperm head. We also demonstrate the existence of β4 integrin mRNAs in round spermatids and spermatogonia by q-RT-PCR, which was further supported by sequencing the PCR products. Using super-resolution microscopy accompanied by colocalization analysis, we located integrin subunits as follows: α6/β4-inner apical acrosomal membrane and equatorial segment; α3, α6/β1, β4-plasma membrane overlaying the apical acrosome; and α3/β1-outer acrosomal m...Continue Reading

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Citations

May 3, 2020·International Journal of Molecular Sciences·Fernando J Peña
May 28, 2020·International Journal of Molecular Sciences·Maria E TevesPaulene Sapao
May 20, 2020·Medical Microbiology and Immunology·Jana JankovičováJana Antalíková
Nov 15, 2020·International Journal of Molecular Sciences·Virginie Barraud-LangeAhmed Ziyyat

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Methods Mentioned

BETA
Assay
PCR
super resolution microscopy
super-resolution microscopy
Electrophoresis
confocal microscopy

Software Mentioned

PYMOL
JACoP ( Just Another Colocalization Plugin )
TASSER
Huygens Professional
GROMACS
PLA
OpenMM Zephyr interface
ClusPro
Fiji
Huygens

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