Adenine, guanine, and inosine nucleotides of chick growth cartilage: relationship between energy status and the mineralization process

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
H MatsumotoI M Shapiro

Abstract

The major aim of this investigation was to measure the nucleotide content of the developing chick epiphysis and to relate changes in nucleotide levels to chondrocyte maturation and the development of mineralization. Using a cryostat, sections of cartilage were isolated from the proximal head of the tibial growth cartilage, care being taken to preserve the metabolic integrity of the tissue. Sections were identified microscopically, pooled, and the nucleotide and nucleoside content of each sample determined by HPLC. Procedures used for the study were shown to minimize degradation of nucleotides. Their effectiveness was assessed through an evaluation of the rapid freezing technique and by examination of the effects of apatite on the recovery of endogenous and added nucleotides. Analysis of nucleotide levels in the growth cartilage indicated that chondrocytes undergo a profound change in energy metabolism during development and maturation. Thus, in the premineralized resting and proliferative zones, ATP and, to a lesser extent, GTP values were high, suggesting that the chondrocytes obtained metabolic energy through both glycolytic and mitochondrial oxidative processes. In the hypertrophic zone and in calcified cartilage, there was ...Continue Reading

References

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Oct 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S KakutaI M Shapiro
May 1, 1985·Calcified Tissue International·S KakutaI M Shapiro
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Citations

Oct 16, 2009·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·Jolene BohenskyIrving M Shapiro
Dec 1, 1995·The International Journal of Biochemistry & Cell Biology·H H Hsu, H C Anderson
Aug 27, 2005·Mitochondrion·Robert TerkeltaubSoumitra Ghosh
May 31, 2016·Joint, Bone, Spine : Revue Du Rhumatisme·Yong HuPascal Reboul
Dec 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M HatoriI M Shapiro
Aug 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·I M ShapiroM Pacifici
Jan 12, 2019·Critical Reviews in Food Science and Nutrition·Bruno BurlandoLaura Cornara
Oct 23, 1997·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K OhyamaI M Shapiro
Aug 23, 2006·Clinical Orthopaedics and Related Research·Nomaan AshrafRalph A Meyer
May 6, 1999·Journal of Cellular Physiology·K MansfieldI M Shapiro
Mar 1, 1996·Calcified Tissue International·A L BoskeyI Binderman
May 13, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G M HobsonR S Tuan
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Cristina C TeixeiraIrving M Shapiro
Aug 1, 1996·Journal of Cellular Physiology·R RajpurohitI M Shapiro
Oct 15, 1991·Biochemical and Biophysical Research Communications·P PolleselloB J Kvam

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