PMID: 8589735Sep 1, 1995Paper

Adenine methylation at dam sites increases transient gene expression in plant cells

Transgenic Research
M W Graham, P J Larkin

Abstract

Escherichia coli encodes two major DNA methylation systems: dam, which produces 6-methyladenine; and dcm, which produces 5-methylcytosine. About 1-2% of adenine and cytosine residues in plasmid DNAs prepared in E. coli are methylated by these systems. Since DNA methylation profoundly influences gene expression in eukaryotes, we were interested in determining whether these bacterially encoded modifications might also effect plant gene expression in experimental systems. We therefore examined the influence of dam and dcm methylation on gene expression from four GUS fusion constructs in transient assays in protoplasts and microprojectile-bombarded whole tissues. In these constructs, GUS expression was driven by promoter regions derived from the Arabidopsis alcohol dehydrogenase (Adh1), maize ubiquitin (Ubi1), rice actin (Act1) and CaMV 35S genes. We show that methyladenine produced by dam methylation increased gene expression from constructs based on the Adh1, Ubi1 and Act1 genes. The increase in gene expression ranged from three-fold for Ubi1 and Adh1 in protoplasts to 50-fold for Act1 in bombarded wheat tissues. Expression of a 35S.GUS construct was, however, insensitive to dam methylation. dcm methylation had little if any effe...Continue Reading

References

Apr 11, 1992·Nucleic Acids Research·M TrussM Beato
Dec 1, 1990·Plant Molecular Biology·L B Farrell, R N Beachy
Sep 30, 1987·Biochemical and Biophysical Research Communications·J A Pintor-Toro
May 27, 1994·Gene·B R Palmer, M G Marinus
Jul 1, 1992·Plant Cell Reports·J J FinerM D McMullen
May 1, 1991·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D I LastE S Dennis

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Citations

Dec 1, 2006·Plant Biotechnology Journal·Yuchan ZhouMichael W Graham
Feb 16, 2006·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·David RatelDidier Wion
Apr 12, 2002·FEBS Letters·Larisa I Fedoreyeva, Boris F Vanyushin
Dec 22, 2019·Molecular Plant·Zhe LiangXiaofeng Gu
May 10, 2011·Biochimica Et Biophysica Acta·Boris F Vanyushin, Vasili V Ashapkin

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