Adeno associated viral-mediated intraosseous labeling of bone marrow derived cells for CNS tracking

Journal of Immunological Methods
Maj-Linda B SelenicaDaniel C Lee

Abstract

Inflammation, including microglial activation in the CNS, is an important hallmark in many neurodegenerative diseases. Microglial stimuli not only impact the brain microenvironment by production and release of cytokines and chemokines, but also influence the activity of bone marrow derived cells and blood born macrophage populations. In many diseases including brain disorders and spinal cord injury, researchers have tried to harbor the neuroprotective and repair properties of these subpopulations. Hematopoietic bone marrow derived cells (BMDCs) are of great interest, especially during gene therapy because certain hematopoietic cell subpopulations traffic to the sites of injury and inflammation. The aim of this study was to develop a method of labeling endogenous bone marrow derived cells through intraosseous impregnation of recombinant adeno-associated virus (rAAV) or lentivirus. We utilized rAAV serotype 9 (rAAV-9) or lentivirus for gene delivery of green florescence protein (GFP) to the mouse bone marrow cells. Flow cytometry showed that both viruses were able to efficiently transduce mouse bone marrow cells in vivo. However, the rAAV9-GFP viral construct transduced BMDCs more efficiently than the lentivirus (11.2% vs. 6.8%),...Continue Reading

References

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May 2, 2012·Fluids and Barriers of the CNS·Angela E SchellenbergJames Peeling
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Nicholas H VarvelMathias Jucker
Jul 23, 2013·Journal of Neuroinflammation·Maj-Linda B SelenicaMarcia N Gordon

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Citations

Jun 19, 2021·Signal Transduction and Targeted Therapy·Hong-Yan ZhaoXiao-Jun Huang

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